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Analytical performance of rapid nucleic acid detection assays and routine RT-qPCR assays for detection of SARS-CoV-2 in Shanghai, China in 2022.
Jiang, Min; Chen, Weiqin; Chen, Yong; Chen, Jia; Zhang, Yue; Yin, Hongmei; Li, Yi; Liu, Weiwei.
  • Jiang M; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Laboratory Medicine and Central Laboratory, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
  • Chen W; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • Chen Y; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • Chen J; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • Zhang Y; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • Yin H; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • Li Y; Department of Nephropathy, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address: liyi_1313@126.com.
  • Liu W; Department of Laboratory Medicine, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address: huashanvivian@126.com.
Diagn Microbiol Infect Dis ; 105(2): 115860, 2022 Nov 12.
Article in English | MEDLINE | ID: covidwho-2231462
ABSTRACT
Diagnostic accuracy of COVID-19 varies among different assays. In this study, the analytical performance of 1 rapid nucleic acid detection assay (Coyote assay) and 2 routine RT-qPCR assays (BioGerm assay and DaAn assay) was evaluated, using 1196 clinical samples. Disagreement in the results of 2 paired targets occurred in all 3 assays. The Coyote assay failed to detect 15 samples, and the DaAn assay failed to detect 5 samples. The Cohen's kappa coefficient was 0.970 between the BioGerm and DaAn assays, 0.907 between the Coyote and BioGerm assays, and 0.936 between the Coyote and DaAn assays. The positive percent agreement, and negative percent agreement of the Coyote assay were 84.04%, and 100%, respectively. Our study revealed that the results of the Coyote, BioGerm, and DaAn assays were highly consistent, which provided reference for the application of these assays for diagnosis of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article Affiliation country: J.diagmicrobio.2022.115860

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: Diagn Microbiol Infect Dis Year: 2022 Document Type: Article Affiliation country: J.diagmicrobio.2022.115860