Shilajit : Extraction, Enrichment & Formulation

ABSTRACT

Incidences and mortality from cardiovascular illnesses have grown during the last few decades. In patients with pre-existing cardiac disease, the COVID-19 pandemic has exacerbated cardiovascular problems. Researchers observed that rates of many conditions, comprising coronary heart failure and stroke, had been drastically higher in those who had recovered from COVID-19 as compared to those who did not have the disease. Herbal medicine is becoming more and more popular due to its relatively high safety and low toxicity. Shilajit has several beneficial properties because it primarily comprises fulvic acid and more than 84 minerals. It demonstrates antioxidant activity, which is a key factor in cardioprotection. Shilajit is appropriately referred to as "Rasayana/ Rasayanam";it translates as "rejuvenator" in Ayurvedic and Siddha scriptures since it prevents illnesses and improves the quality of life. The current study involved the collection, authentication, purification, and use of several extractive solvents to achieve high yield, then the extract was enriched by using organic solvents. UV-Vis spectroscopy was performed on each of the enriched samples, and the results showed that the enriched samples had increased fulvic acid concentrations. Fulvic acid was used as a standard in thin-layer chromatography. The extract's in-vitro antioxidant activity was assessed using the DPPH assay, and the results indicated good antioxidant activity. phytosomes were formulated by rapid injection methods for extract and enriched extract, and they were characterized using several spectroscopic techniques. According to the study's findings, combining novel formulation with conventional drug may improve its therapeutic potential and bioavailability.