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Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern.
Morgan, Mahali S; Yan, Kexin; Le, Thuy T; Johnston, Ryan A; Amarilla, Alberto A; Muller, David A; McMillan, Christopher L D; Modhiran, Naphak; Watterson, Daniel; Potter, James R; Sng, Julian D J; Lor, Mary; Paramitha, Devina; Isaacs, Ariel; Khromykh, Alexander A; Hall, Roy A; Suhrbier, Andreas; Rawle, Daniel J; Hobson-Peters, Jody.
  • Morgan MS; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Yan K; Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
  • Le TT; Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
  • Johnston RA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Amarilla AA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Muller DA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • McMillan CLD; Global Virus Network Centre of Excellence, Australian Infectious Diseases Research Centre, Brisbane, QLD 4072 and 4029, Australia.
  • Modhiran N; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Watterson D; Global Virus Network Centre of Excellence, Australian Infectious Diseases Research Centre, Brisbane, QLD 4072 and 4029, Australia.
  • Potter JR; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Sng JDJ; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Lor M; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Paramitha D; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Isaacs A; Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia.
  • Khromykh AA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Hall RA; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Suhrbier A; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
  • Rawle DJ; Global Virus Network Centre of Excellence, Australian Infectious Diseases Research Centre, Brisbane, QLD 4072 and 4029, Australia.
  • Hobson-Peters J; School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia.
Viruses ; 15(1)2022 Dec 31.
Article in English | MEDLINE | ID: covidwho-2233467
ABSTRACT
The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the reactivity to multiple variants of concern using ELISAs and describe the use of the antibodies in indirect immunofluorescence assays, Western blots, and rapid antigen tests. Finally, we illustrate the ability of two antibodies to reduce significantly viral tissue titers in K18-hACE2 transgenic mice infected with the original and an omicron isolate of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Antibodies, Monoclonal Topics: Variants Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V15010139

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Antibodies, Monoclonal Topics: Variants Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: V15010139