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Experimental and clinical data analysis for identification of COVID-19 resistant ACE2 mutations.
Raghav, Pawan Kumar; Raghav, Aditya; Lathwal, Anjali; Saxena, Archit; Mann, Zoya; Sengar, Manisha; Rajalingam, Raja.
  • Raghav PK; Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA, USA. PwnRghv@gmail.com.
  • Raghav A; BioExIn, Delhi, India.
  • Lathwal A; Department of Computational Biology, Indraprastha Institute of Information Technology, New Delhi, India.
  • Saxena A; Amity Institute of Biotechnology, Amity University, Sector-125, Noida, Uttar Pradesh, India.
  • Mann Z; BioExIn, Delhi, India.
  • Sengar M; Department of Zoology, Deshbandhu College, University of Delhi, Delhi, India.
  • Rajalingam R; Immunogenetics and Transplantation Laboratory, Department of Surgery, University of California San Francisco, San Francisco, CA, USA.
Sci Rep ; 13(1): 2351, 2023 02 09.
Article in English | MEDLINE | ID: covidwho-2234671
ABSTRACT
The high magnitude zoonotic event has caused by Severe Acute Respitarory Syndrome CoronaVirus-2 (SARS-CoV-2) is Coronavirus Disease-2019 (COVID-19) epidemics. This disease has high rate of spreading than mortality in humans. The human receptor, Angiotensin-Converting Enzyme 2 (ACE2), is the leading target site for viral Spike-protein (S-protein) that function as binding ligands and are responsible for their entry in humans. The patients infected with COVID-19 with comorbidities, particularly cancer patients, have a severe effect or high mortality rate because of the suppressed immune system. Nevertheless, there might be a chance wherein cancer patients cannot be infected with SARS-CoV-2 because of mutations in the ACE2, which may be resistant to the spillover between species. This study aimed to determine the mutations in the sequence of the human ACE2 protein and its dissociation with SARS-CoV-2 that might be rejecting viral transmission. The in silico approaches were performed to identify the impact of SARS-CoV-2 S-protein with ACE2 mutations, validated experimentally, occurred in the patient, and reported in cell lines. The identified changes significantly affect SARS-CoV-2 S-protein interaction with ACE2, demonstrating the reduction in the binding affinity compared to SARS-CoV. The data presented in this study suggest ACE2 mutants have a higher and lower affinity with SARS-Cov-2 S-protein to the wild-type human ACE2 receptor. This study would likely be used to report SARS-CoV-2 resistant ACE2 mutations and can be used to design active peptide development to inactivate the viral spread of SARS-CoV-2 in humans.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2023 Document Type: Article Affiliation country: S41598-022-20773-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2023 Document Type: Article Affiliation country: S41598-022-20773-9