Present status of microfluidic PCR chip in nucleic acid detection and future perspective
TrAC - Trends in Analytical Chemistry
; 157 (no pagination), 2022.
Article
in English
| EMBASE | ID: covidwho-2236003
ABSTRACT
Polymerase chain reaction (PCR) amplifies specific fragment of DNA molecules and has been extensively applied in fields of pathogens and gene mutation detection, food safety and clinical diagnosis which on the other hand, holds the drawbacks of large size instrument, high heat dissipation etc. It has been demonstrated that microfluidics technique coupling with PCR reaction exhibits characteristics of integration, automatization, miniaturization, and portability. Meanwhile, various designed fabrication of microchip could contribute to diverse applications. In this review, we summarized major works about a variety of microfluidic chips equipped with several kinds of PCR techniques (PCR, RT-PCR, mPCR, dPCR) and detection methods like fluorescence, electrochemistry, and electrophoresis detection. The development and application of PCR-based microfluidic chip in pathogen and gene mutation detection, diseases prevention and diagnosis, DNA hybridization and low-volume sample treatment were also discussed. Copyright © 2022 Elsevier B.V.
Future direction; Integration; Microfluidic chip; Nucleic acid detection; PCR techniques; automation; breast cancer; Brucella; DNA extraction; DNA hybridization; DNA methylation; DNA sequencing; droplet digital polymerase chain reaction; electrochemistry; electrophoresis; Escherichia coli; fluorescence; gene identification; gene mutation; genotyping; high throughput sequencing; human; infectious agent; lab on a chip; ligation; microfluidics; microorganism detection; miniaturization; multiplex polymerase chain reaction; non small cell lung cancer; nonhuman; nucleic acid analysis; periodontitis; polymerase chain reaction; prostate cancer; real time polymerase chain reaction; review; Salmonella; SARS coronavirus; serpentine; DNA microarray kit; proximity ligation assay
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Type of study:
Prognostic study
Language:
English
Journal:
TrAC - Trends in Analytical Chemistry
Year:
2022
Document Type:
Article
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