Advances in droplet digital polymerase chain reaction on microfluidic chips.
Lab Chip
; 23(5): 1258-1278, 2023 03 01.
Article
in English
| MEDLINE | ID: covidwho-2237144
ABSTRACT
The PCR technique has been known to the general public since the pandemic outbreak of COVID-19. This technique has progressed through three stages from simple PCR to real-time fluorescence PCR to digital PCR. Among them, the microfluidic-based droplet digital PCR technique has attracted much attention and has been widely applied due to its advantages of high throughput, high sensitivity, low reagent consumption, low cross-contamination, and absolute quantification ability. In this review, we introduce various designs of microfluidic-based ddPCR developed within the last decade. The microfluidic-based droplet generation methods, thermal cycle strategies, and signal counting approaches are described, and the applications in the fields of single-cell analysis, disease diagnosis, and pathogen detection are introduced. Further, the challenges and prospects of microfluidic-based ddPCR are discussed. We hope that this review can contribute to the further development of the microfluidic-based ddPCR technique.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Microfluidics
/
COVID-19
Type of study:
Diagnostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
Lab Chip
Journal subject:
Biotechnology
/
Chemistry
Year:
2023
Document Type:
Article
Affiliation country:
D2lc00814a
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