Your browser doesn't support javascript.
Advances in droplet digital polymerase chain reaction on microfluidic chips.
Xu, Danfeng; Zhang, Weifei; Li, Hongmei; Li, Nan; Lin, Jin-Ming.
  • Xu D; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China. zhangweifei@nim.ac.cn.
  • Zhang W; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China. zhangweifei@nim.ac.cn.
  • Li H; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China. zhangweifei@nim.ac.cn.
  • Li N; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), China. jmlin@mail.tsinghua.edu.cn.
  • Lin JM; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), China. jmlin@mail.tsinghua.edu.cn.
Lab Chip ; 23(5): 1258-1278, 2023 03 01.
Article in English | MEDLINE | ID: covidwho-2237144
ABSTRACT
The PCR technique has been known to the general public since the pandemic outbreak of COVID-19. This technique has progressed through three stages from simple PCR to real-time fluorescence PCR to digital PCR. Among them, the microfluidic-based droplet digital PCR technique has attracted much attention and has been widely applied due to its advantages of high throughput, high sensitivity, low reagent consumption, low cross-contamination, and absolute quantification ability. In this review, we introduce various designs of microfluidic-based ddPCR developed within the last decade. The microfluidic-based droplet generation methods, thermal cycle strategies, and signal counting approaches are described, and the applications in the fields of single-cell analysis, disease diagnosis, and pathogen detection are introduced. Further, the challenges and prospects of microfluidic-based ddPCR are discussed. We hope that this review can contribute to the further development of the microfluidic-based ddPCR technique.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Microfluidics / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: Lab Chip Journal subject: Biotechnology / Chemistry Year: 2023 Document Type: Article Affiliation country: D2lc00814a

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Microfluidics / COVID-19 Type of study: Diagnostic study / Randomized controlled trials Limits: Humans Language: English Journal: Lab Chip Journal subject: Biotechnology / Chemistry Year: 2023 Document Type: Article Affiliation country: D2lc00814a