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ANGIOTENSIN II EXAGGERATES SARS-COV-2 SPECIFIC T-CELL RESPONSE IN CONVALESCENT INDIVIDUALS FOLLOWING COVID-19
Journal of Hypertension ; 41:e139, 2023.
Article in English | EMBASE | ID: covidwho-2238591
ABSTRACT

Background:

Coronavirus disease 2019 (COVID-19) is an emerging respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARSCoV- 2). Recent studies have suggested numerous hypotheses that may explain multi-organ dysfunction during a COVID-19 infection. One possible hypothesis posits that the renin-angiotensin system dysregulation before SARS-CoV-2 infection could exacerbate disease symptoms and severity, especially in COVID-19 patients with underlying comorbidities.

Objective:

This study sought to investigate the effect of exogenous angiotensin II (Ang II) on peripheral blood mononuclear cells (PBMCs) stimulated with SARSCoV- 2 peptide pool.

Methods:

PBMCs from recovered COVID-19 patients (n = 18) were used in this study. SARS-CoV-2 specific t-cell responses were measured using activation induced cell marker assay and intracellular cytokine staining (ICS) assay, while enzyme-linked immunosorbent assay (ELISA) and ICS assays determined functional capability and polarization. Additionally, the relative level of protein phosphorylation in PBMCs was measured using a phosphokinase array.

Results:

Our results showed that in vitro Ang II treatment significantly increased the magnitude of SARS-CoV-2 specific t-cell response in stimulated PBMCs with SARS-CoV-2 peptide pool. Moreover, the phosphorylation level of numerous proteins implicated in cardiovascular diseases, inflammation, and viral infection showed significant increase in the presence of Ang II. Mitogenic stimulation of PBMCs after Ang II and SARS-CoV-2 peptide pool stimulation showed functional polarization of CD4+ and CD8+ t-cells toward Th1/Th17 and Th17 phenotypes, respectively. Meanwhile, ELISA showed an increased production of IL-1b and IL-6 in Ang II-stimulated PBMCs without affecting the reduction of IL-10 level resulting from SARS-CoV-2 peptide pool stimulation.

Conclusion:

To our knowledge, the present study is the first to demonstrate that Ang II exaggerates SARS-CoV-2 specific t-cells response. Therefore, during COVID-19 infection Ang II may aggravate the inflammatory response and change the immune response toward a more inflammatory profile against SARS-CoV-2 infection, leading to serious complications and worse outcomes during COVID-19 infection.
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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Journal of Hypertension Year: 2023 Document Type: Article

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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Journal of Hypertension Year: 2023 Document Type: Article