Protocol for investigating the biogenesis of SARS-CoV-2 S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodies.
STAR Protoc
; 4(1): 101977, 2022 Dec 15.
Article
in English
| MEDLINE | ID: covidwho-2240057
ABSTRACT
The protocol is designed to investigate the influence of an anti-cleavage site intrabody in modulating the output of LV(CoV-2 S), a lentivirus-based pseudovirus expressing CoV-2 S protein using HEK293T cells. We clone the single-domain antibody sequence into a lentiviral vector (pLenti-GFP) for intracellular expression and assess not only the viral biogenesis but also the fate of the CoV-2 S protein in such cells. For complete details on the use and execution of this protocol, please refer to Singh et al. (2022).1.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
STAR Protoc
Year:
2022
Document Type:
Article
Affiliation country:
J.xpro.2022.101977
Similar
MEDLINE
...
LILACS
LIS