Generation of APN-chimeric gene-edited pigs by CRISPR/Cas9-mediated knock-in strategy
Gene
; 851, 2023.
Article
in English
| Scopus | ID: covidwho-2242821
ABSTRACT
The prevalence of porcine enteric coronaviruses (PECs), including transmissible gastroenteritis virus (TGEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine delta coronavirus (PDCoV), and porcine epidemic diarrhea virus (PEDV), poses a serious threat to animal and public health. Here, we aimed to further optimize the porcine aminopeptidase N (pAPN) gene editing strategy to explore the balance between individual antiviral properties and the biological functions of pAPN in pigs. Finally, APN-chimeric gene-edited pigs were produced through a CRISPR/Cas9-mediated knock-in strategy. Further reproductive tests indicated that these gene-edited pigs exhibited normal pregnancy rates and viability. Notably, in vitro viral challenge assays further demonstrated that porcine kidney epithelial cells isolated from F1-generation gene-edited pigs could effectively inhibit TGEV infection. This study is the first to report the generation of APN-chimeric pigs, which may provide a natural host animal for characterizing PEC infection with APN and help in the development of better antiviral solutions. © 2022 Elsevier B.V.
Animals; Antiviral Agents; Coronavirus Infections; CRISPR-Cas Systems; Gene Editing; Porcine epidemic diarrhea virus; Swine; Swine Diseases; Transmissible gastroenteritis virus; antivirus agent; animal; Coronavirus infection; CRISPR Cas system; genetics; pig; swine disease; veterinary medicine; APN; CRISPR/Cas9; Gene-edited pigs; Knock-in; TGEV
Full text:
Available
Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Gene
Year:
2023
Document Type:
Article
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