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Molecular imaging of chemokine-like receptor 1 (CMKLR1) in experimental acute lung injury.
Mannes, Philip Z; Barnes, Clayton E; Biermann, Jana; Latoche, Joseph D; Day, Kathryn E; Zhu, Qin; Tabary, Mohammadreza; Xiong, Zeyu; Nedrow, Jessie R; Izar, Benjamin; Anderson, Carolyn J; Villanueva, Flordeliza S; Lee, Janet S; Tavakoli, Sina.
  • Mannes PZ; Department of Radiology, University of Pittsburgh, Pittsburgh 15213, PA.
  • Barnes CE; Medical Scientist Training Program, University of Pittsburgh, Pittsburgh 15261, PA.
  • Biermann J; Department of Radiology, University of Pittsburgh, Pittsburgh 15213, PA.
  • Latoche JD; Department of Medicine, Division of Hematology/Oncology, Columbia University Irving Medical Center, New York 10032, NY.
  • Day KE; Program for Mathematical Genomics, Columbia University, New York 10032, NY.
  • Zhu Q; Department of Medicine, University of Pittsburgh, Pittsburgh 15213, PA.
  • Tabary M; Department of Medicine, University of Pittsburgh, Pittsburgh 15213, PA.
  • Xiong Z; Department of Radiology, University of Pittsburgh, Pittsburgh 15213, PA.
  • Nedrow JR; Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, Pittsburgh 15213, PA.
  • Izar B; Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, Pittsburgh 15213, PA.
  • Anderson CJ; Department of Radiology, University of Pittsburgh, Pittsburgh 15213, PA.
  • Villanueva FS; Department of Medicine, Division of Hematology/Oncology, Columbia University Irving Medical Center, New York 10032, NY.
  • Lee JS; Program for Mathematical Genomics, Columbia University, New York 10032, NY.
  • Tavakoli S; Department of Chemistry, University of Missouri, Columbia 65211, MO.
Proc Natl Acad Sci U S A ; 120(3): e2216458120, 2023 01 17.
Article in English | MEDLINE | ID: covidwho-2243078
ABSTRACT
The lack of techniques for noninvasive imaging of inflammation has challenged precision medicine management of acute respiratory distress syndrome (ARDS). Here, we determined the potential of positron emission tomography (PET) of chemokine-like receptor-1 (CMKLR1) to monitor lung inflammation in a murine model of lipopolysaccharide-induced injury. Lung uptake of a CMKLR1-targeting radiotracer, [64Cu]NODAGA-CG34, was significantly increased in lipopolysaccharide-induced injury, correlated with the expression of multiple inflammatory markers, and reduced by dexamethasone treatment. Monocyte-derived macrophages, followed by interstitial macrophages and monocytes were the major CMKLR1-expressing leukocytes contributing to the increased tracer uptake throughout the first week of lipopolysaccharide-induced injury. The clinical relevance of CMKLR1 as a biomarker of lung inflammation in ARDS was confirmed using single-nuclei RNA-sequencing datasets which showed significant increases in CMKLR1 expression among transcriptionally distinct subsets of lung monocytes and macrophages in COVID-19 patients vs. controls. CMKLR1-targeted PET is a promising strategy to monitor the dynamics of lung inflammation and response to anti-inflammatory treatment in ARDS.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Distress Syndrome / Acute Lung Injury / COVID-19 Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Proc Natl Acad Sci U S A Year: 2023 Document Type: Article Affiliation country: Pnas.2216458120

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Distress Syndrome / Acute Lung Injury / COVID-19 Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Proc Natl Acad Sci U S A Year: 2023 Document Type: Article Affiliation country: Pnas.2216458120