Baicalin Ameliorates Preeclampsia In Vitro by Regulating the miRNA-19a/PTEN Axis

ABSTRACT

Baicalin is a kind of extraction from herb, and had treatment effects in some disease, however, it has been unclear that it's effects in preeclampsia (PE). The aim of our work was to evaluate baicalin's effects in PE treatment and relative mechanisms in vivo. Using hypoxia to make PE cell model. First step, using difference baicalin concentration to treat. Next step, transfecting si-miRNA-19a to discuss miRNA-19a's effects in baicalin's treatment to PE. Measuring cell proliferation, apoptosis, invasion and migration by CCK-8, flow cytometer, transwell and wound healing assay. Relative protein and gene expression by WB and RT-qPCR assay. Analysis correlation between miRNA-19a and PTEN by dual-luciferase reporter gene assay. Compared with NC, cell proliferation was significantly depressed with apoptosis significantly increasing and invasion cell number and wound healing rates were significantly down-regulation. miRNA-19a expression was significantly down-regulation, PTEN expression was significantly up-regulation, and p-AKT and p-PI3K expressions were significantly down-regulation. With baicalin supplement, the cell's biological activities including cell proliferation, invasion and migration were significantly up-regulation with miRNA-19a increasing. Meanwhile, PTEN protein expression was significantly depressed and p-AKT and p-PI3K proteins expression were significantly increased (p < 0.001, respectively). By dual-luciferase reporter gene assay, miRNA-19a could target PTEN in cell lines. Baicalin had effects to improve PE with miRNA-19a/PTEN axis in vivo study.