Hybrid Peptide–Agarose Hydrogels for 3D Immunoassays
Methods in Molecular Biology
; 2578:53-62, 2023.
Article
in English
| Scopus | ID: covidwho-2243611
ABSTRACT
Recent advances in biosensing analytical platforms have brought relevant outcomes for novel diagnostic and therapy-oriented applications. In this context, 3D droplet microarrays, where hydrogels are used as matrices to stably entrap biomolecules onto analytical surfaces, potentially provide relevant advantages over conventional 2D assays, such as increased loading capacity, lower nonspecific binding, and enhanced signal-to-noise ratio. Here, we describe a hybrid hydrogel composed of a self-assembling peptide and commercial agarose (AG) as a suitable matrix for 3D microarray bioassays. The hybrid hydrogel is printable and self-adhesive and allows analyte diffusion. As a showcase example, we describe its application in a diagnostic immunoassay for the detection of SARS-CoV-2 infection. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
COVID-19; Humans; Hydrogels; Immunoassay; Peptides; Resin Cements; SARS-CoV-2; Sepharose; agarose; hydrogel; monomer; peptide; resin; resin cement; controlled study; coronavirus disease 2019; diffusion; human; microarray analysis; nonhuman; peptide synthesis; protein purification; reversed phase high performance liquid chromatography; chemistry; diagnosis; 3D bioassays; Microarrays; Self-assembly peptide hydrogels; Supramolecular nanostructures
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Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Methods in Molecular Biology
Year:
2023
Document Type:
Article
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