Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.
NPJ Vaccines ; 8(1): 18, 2023 Feb 14.
Article in English | MEDLINE | ID: covidwho-2243664
ABSTRACTDevelopment of SARS-CoV-2 vaccines that protect vulnerable populations is a public health priority. Here, we took a systematic and iterative approach by testing several adjuvants and SARS-CoV-2 antigens to identify a combination that elicits antibodies and protection in young and aged mice. While demonstrating superior immunogenicity to soluble receptor-binding domain (RBD), RBD displayed as a protein nanoparticle (RBD-NP) generated limited antibody responses. Comparison of multiple adjuvants including AddaVax, AddaS03, and AS01B in young and aged mice demonstrated that an oil-in-water emulsion containing carbohydrate fatty acid monosulphate derivative (CMSO/W) most effectively enhanced RBD-NP-induced cross-neutralizing antibodies and protection across age groups. CMSO/W enhanced antigen retention in the draining lymph node, induced injection site, and lymph node cytokines, with CMS inducing MyD88-dependent Th1 cytokine polarization. Furthermore, CMS and O/W synergistically induced chemokine production from human PBMCs. Overall, CMSO/W adjuvant may enhance immunogenicity and protection of vulnerable populations against SARS-CoV-2 and other infectious pathogens.
Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Randomized controlled trials / Systematic review/Meta Analysis Topics: Vaccines Language: English Journal: NPJ Vaccines Year: 2023 Document Type: Article Affiliation country: S41541-023-00610-4