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Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein.
Niu, Xiaoyu; Xu, Jiayu; Liu, Mingde; Tu, Huolin; Koenig, Sara N; Saif, Linda J; Jones, Daniel M; Wang, Qiuhong.
  • Niu X; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
  • Xu J; Center for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
  • Liu M; Department of Epidemiology, University of North Carolina At Chapel Hill, Chapel Hill, NC, 27516, USA.
  • Tu H; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
  • Koenig SN; Center for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
  • Saif LJ; Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
  • Jones DM; Center for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
  • Wang Q; James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA.
Arch Virol ; 168(1): 5, 2022 Dec 20.
Article in English | MEDLINE | ID: covidwho-2243796
ABSTRACT
We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020-January 2021.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Topics: Variants Limits: Humans Language: English Journal: Arch Virol Year: 2022 Document Type: Article Affiliation country: S00705-022-05621-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Topics: Variants Limits: Humans Language: English Journal: Arch Virol Year: 2022 Document Type: Article Affiliation country: S00705-022-05621-5