Diagnostic test property of transcription-reverse transcription concerted reaction reagent TRCReady® SARS-CoV-2 i using nasopharyngeal swab samples
Journal of Infection and Chemotherapy
; 29(1):115-117, 2023.
Article
in English
| Scopus | ID: covidwho-2243859
ABSTRACT
TRCReady® SARS-CoV-2 i is a reagent for transcription-reverse transcription concerted reaction (TRC) to detect SARS-CoV-2 N2 gene, used with the automated rapid isothermal nucleic acid amplification test (NAAT) analyzer TRCReady®-80. Sensitivity and specificity of TRCReady® SARS-CoV-2 i was assessed by comparison with the results of real-time reverse transcription-polymerase chain reaction (RT-PCR) using nasopharyngeal swab samples. From November 2020 to March 2021, a total of 441 nasopharyngeal swabs were obtained and analyzed both with TRCReady® SARS-CoV-2 i and RT-PCR. Sensitivity and specificity of TRCReady® SARS-CoV-2 i were 94.6% (53/56) and 99.2% (382/385), respectively. Reaction time to positivity of TRCReady® SARS-CoV-2 i ranged from 1.166 to 9.805 (median 2.887) min, and minimum detection sensitivity of TRCReady® SARS-CoV-2 i was 9 copies per test, with reaction time as 5.014 min. Detection of SARS-CoV-2 gene from nasopharyngeal swab sample using TRCReady® SARS-CoV-2 i shows comparative diagnostic test accuracy with RT-PCR, and can be used as a useful test to diagnose SARS-CoV-2 infection. © 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases
COVID-19; Diagnostic Tests, Routine; Humans; Indicators and Reagents; Nasopharynx; Reverse Transcription; SARS-CoV-2; Sensitivity and Specificity; reagent; trcready sars cov 2i; dyes, reagents, indicators, markers and buffers; Article; controlled study; coronavirus disease 2019; diagnostic accuracy; diagnostic test accuracy study; human; human tissue; intermethod comparison; isothermal amplification; nasopharyngeal swab; reaction time; real time reverse transcription polymerase chain reaction; Severe acute respiratory syndrome coronavirus 2; standard; transcription reverse transcription concerted reaction; virus gene; diagnosis; diagnostic test; genetics; Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Rapid nucleic acid amplification test; Transcription-reverse transcription concerted reaction; TRCReady® SARS-CoV-2 i
Full text:
Available
Collection:
Databases of international organizations
Database:
Scopus
Type of study:
Diagnostic study
Language:
English
Journal:
Journal of Infection and Chemotherapy
Year:
2023
Document Type:
Article
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