A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic.
Arch Virol
; 168(3): 87, 2023 Feb 14.
Article
in English
| MEDLINE | ID: covidwho-2244493
ABSTRACT
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Influenza A virus
/
Respiratory Tract Infections
/
RNA Viruses
/
Respiratory Syncytial Virus, Human
/
Influenza, Human
/
Coinfection
/
COVID-19
Type of study:
Diagnostic study
/
Observational study
Limits:
Adult
/
Child
/
Humans
Language:
English
Journal:
Arch Virol
Year:
2023
Document Type:
Article
Affiliation country:
S00705-023-05717-6
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