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A high-throughput fully automatic biosensing platform for efficient COVID-19 detection.
Rong, Guoguang; Zheng, Yuqiao; Li, Xiangqing; Guo, Mengzhun; Su, Yi; Bian, Sumin; Dang, Bobo; Chen, Yin; Zhang, Yanjun; Shen, Linhai; Jin, Hui; Yan, Renhong; Wen, Liaoyong; Zhu, Peixi; Sawan, Mohamad.
  • Rong G; CenBRAIN Neurotech, School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced
  • Zheng Y; CenBRAIN Neurotech, School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced
  • Li X; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, 310024, China.
  • Guo M; Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake, University, Hangzhou, Zhejiang, 310030, China; Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310030, China; Institute of Biology, Westlake
  • Su Y; CenBRAIN Neurotech, School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced
  • Bian S; CenBRAIN Neurotech, School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced
  • Dang B; Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake, University, Hangzhou, Zhejiang, 310030, China; Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310030, China; Institute of Biology, Westlake
  • Chen Y; Zhejiang Provincial Center for Disease Control and Prevention, 3399 Binsheng Road, Hangzhou, Zhejiang, 310051, China.
  • Zhang Y; Zhejiang Provincial Center for Disease Control and Prevention, 3399 Binsheng Road, Hangzhou, Zhejiang, 310051, China.
  • Shen L; Hangzhou Center for Disease Control and Prevention, 568 Mingshi Road, Jianggan District, Hangzhou, Zhejiang, 310021, China.
  • Jin H; Hangzhou Center for Disease Control and Prevention, 568 Mingshi Road, Jianggan District, Hangzhou, Zhejiang, 310021, China.
  • Yan R; Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake, University, Hangzhou, Zhejiang, 310030, China; Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310030, China.
  • Wen L; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced Study, Hangzhou, Zhejiang, 310024, China.
  • Zhu P; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.
  • Sawan M; CenBRAIN Neurotech, School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; School of Engineering, Westlake University, 600 Dunyu Road, Xihu District, Hangzhou, Zhejiang, 310030, China; Institute of Advanced Study, Westlake Institute for Advanced
Biosens Bioelectron ; 220: 114861, 2023 Jan 15.
Article in English | MEDLINE | ID: covidwho-2244685
ABSTRACT
We propose a label-free biosensor based on a porous silicon resonant microcavity and localized surface plasmon resonance. The biosensor detects SARS-CoV-2 antigen based on engineered trimeric angiotensin converting enzyme-2 binding protein, which is conserved across different variants. Robotic arms run the detection process including sample loading, incubation, sensor surface rinsing, and optical measurements using a portable spectrometer. Both the biosensor and the optical measurement system are readily scalable to accommodate testing a wide range of sample numbers. The limit of detection is 100 TCID50/ml. The detection time is 5 min, and the throughput of one single robotic site is up to 384 specimens in 30 min. The measurement interface requires little training, has standard operation, and therefore is suitable for widespread use in rapid and onsite COVID-19 screening or surveillance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Optical Devices / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Optical Devices / COVID-19 Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2023 Document Type: Article