COI vs Ct: comparing automated antigen tests cut-off index (COI) to PCR cycle threshold (Ct)
Diagnostic Microbiology and Infectious Disease
; 105(1), 2023.
Article
in English
| Scopus | ID: covidwho-2245935
ABSTRACT
Introduction:
The objective of this study was to compare the numeric cut-off index (COI) of automated antigen tests with the cycle-threshold (Ct) value. Materials andmethods:
COI values of all samples processed with the Elecsys® SARS-CoV-2 Antigen (Roche, Switzerland) from January to February 2022 were retrieved and the positive were compared to RdRP Cts of Allplex Variants I (Seegene, Korea). COI between 0.6 and 1 were considered indeterminate and overproved by RT-PCR.Results:
From 13,937 samples 7944(57%) were positive and 189(1.35%) indeterminate. There was a strong correlation between Cts and COI values at the positive samples, but that was not the case for indeterminates.Conclusions:
COI values of the positive samples (COI >1) are comparable to Ct values and may therefore be used as proxy viral loads, however this is not the case for indeterminate samples. © 2022 Elsevier Inc.
COVID-19; Humans; Immunologic Tests; Polymerase Chain Reaction; Republic of Korea; SARS-CoV-2; Sensitivity and Specificity; analytical parameters; Article; controlled study; coronavirus disease 2019; cut off index value; cycle threshold value; human; nasopharyngeal swab; nucleic acid amplification techniques; real time polymerase chain reaction; SARS-CoV-2 Delta; SARS-CoV-2 Omicron; SARS-CoV-2 Omicron BA.2; virus load; diagnosis; genetics; immunological procedures; South Korea; Antigen tests; Omicron
Full text:
Available
Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Diagnostic Microbiology and Infectious Disease
Year:
2023
Document Type:
Article
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