Your browser doesn't support javascript.
Detection of SARS-CoV-2 IgG antibodies and inflammatory cytokines in saliva-a pilot study.
Wadhwa, S; Yoon, A J; Kister, K; Bolin, I; Chintalapudi, N; Besmer, A; Cantos, A; Shah, J; Gaitonde, S K; Granger, S W; Bryce, C; Fischer, R; Eisig, S B; Yin, M T.
  • Wadhwa S; Division of Orthodontics, Columbia University Irving Medical Center, New York, NY, USA.
  • Yoon AJ; Division of Oral & Maxillofacial Pathology and Department of Pathology & Cell Biology, Columbia University Irving Medical Center, New York, NY, USA.
  • Kister K; Division of Orthodontics, Columbia University Irving Medical Center, New York, NY, USA.
  • Bolin I; Division of Orthodontics, Columbia University Irving Medical Center, New York, NY, USA.
  • Chintalapudi N; Division of Orthodontics, Columbia University Irving Medical Center, New York, NY, USA.
  • Besmer A; Division of Orthodontics, Columbia University Irving Medical Center, New York, NY, USA.
  • Cantos A; Department of Medicine Infectious Disease, Columbia University Irving Medical Center, New York, NY, USA.
  • Shah J; Department of Medicine Infectious Disease, Columbia University Irving Medical Center, New York, NY, USA.
  • Gaitonde SK; Salimetrics, LLC, Carlsbad, CA, USA.
  • Granger SW; Salimetrics, LLC, Carlsbad, CA, USA.
  • Bryce C; Salimetrics, LLC, Carlsbad, CA, USA.
  • Fischer R; Salimetrics, LLC, Carlsbad, CA, USA.
  • Eisig SB; Division of Oral & Maxillofacial Surgery, Columbia University Irving Medical Center, New York, NY, USA.
  • Yin MT; Department of Medicine Infectious Disease, Columbia University Irving Medical Center, New York, NY, USA.
J Oral Biol Craniofac Res ; 13(2): 267-271, 2023.
Article in English | MEDLINE | ID: covidwho-2246125
ABSTRACT

Objective:

The pandemic caused by SARS-CoV-2 virus continues to have a profound effect worldwide. However, COVID-19 induced oral facial manifestations have not been fully described. We conducted a prospective study to demonstrate feasibility of anti-SARS-CoV-2 IgG and inflammatory cytokine detection in saliva. Our primary objective was to determine whether COVID-19 PCR positive patients with xerostomia or loss of taste had altered serum or saliva cytokine levels compared to COVID-19 PCR positive patients without those oral symptoms. Our secondary objective was to determine the correlation between serum and saliva COVID-19 antibody levels. Materials and

methods:

For cytokine analysis, saliva and serum were obtained from 17 participants with PCR-confirmed COVID-19 infection at three sequential time points, yielding 48 saliva samples and 19 paired saliva-serum samples from 14 of the 17 patients. For COVID-19 antibody analyses, an additional 27 paired saliva-serum samples from 22 patients were purchased.

Results:

The saliva antibody assay had 88.64% sensitivity [95% Confidence Interval (CI) 75.44%, 96.21%] to detect SARS-CoV-2 IgG antibodies compared to serum antibody. Among the inflammatory cytokines assessed - IL-6, TNF-α, IFN-γ, IL-10, IL-12p70, IL-1ß, IL-8, IL-13, IL-2, IL-5, IL-7 and IL-17A, xerostomia correlated with lower levels of saliva IL-2 and TNF-α, and elevated levels of serum IL-12p70 and IL-10 (p < 0.05). Loss of taste was observed in patients with elevated serum IL-8 (p < 0.05).

Conclusions:

Further studies are needed to construct a robust saliva-based COVID-19 assay to assess antibody and inflammatory cytokine response, which has potential utility as a non-invasive monitoring modality during COVID-19 convalescence.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Language: English Journal: J Oral Biol Craniofac Res Year: 2023 Document Type: Article Affiliation country: J.jobcr.2023.02.008

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Language: English Journal: J Oral Biol Craniofac Res Year: 2023 Document Type: Article Affiliation country: J.jobcr.2023.02.008