Your browser doesn't support javascript.
COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy.
Zhang, Pei-Pei; He, Zhi-Cheng; Yao, Xiao-Hong; Tang, Rui; Ma, Jie; Luo, Tao; Zhu, Chuhong; Li, Tian-Ran; Liu, Xindong; Zhang, Dingyu; Zhang, Shuyang; Ping, Yi-Fang; Leng, Ling; Bian, Xiu-Wu.
  • Zhang PP; Department of Pathology, the First Affiliated Hospital of University of Science & Technology of China, 230036, Hefei, Anhui, P.R. China.
  • He ZC; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Yao XH; Department of Pathology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 200025, Shanghai, P.R. China.
  • Tang R; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Ma J; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China. yxh16@126.com.
  • Luo T; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Zhu C; Stem Cell and Regenerative Medicine Lab, Department of Medical Science Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Translational Medicine Center, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 100730, Beijin
  • Li TR; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Liu X; Department of Anatomy, Key Laboratory for Biomechanics and Tissue Engineering of Chongqing, State Key Laboratory of Trauma, Burn and Combined injury, Army Medical University (Third Military Medical University), 400038, Chongqing, P.R. China.
  • Zhang D; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Zhang S; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China.
  • Ping YF; Wuhan Jinyintan Hospital (Wuhan Hospital for Infectious Diseases), 430015, Wuhan, Hubei, P.R. China.
  • Leng L; Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 100730, Beijing, P.R. China.
  • Bian XW; Institute of Pathology & Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University), and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, 400038, Chongqing, P.R. China. pingyifang@126.com.
Signal Transduct Target Ther ; 8(1): 24, 2023 01 06.
Article in English | MEDLINE | ID: covidwho-2246724
ABSTRACT
Severe neurological symptoms are associated with Coronavirus disease 2019 (COVID-19). However, the morphologic features, pathological nature and their potential mechanisms in patient brains have not been revealed despite evidence of neurotropic infection. In this study, neuropathological damages and infiltrating inflammatory cells were quantitatively evaluated by immunohistochemical staining, ultrastructural examination under electron microscopy, and an image threshold method, in postmortem brains from nine critically ill COVID-19 patients and nine age-matched cadavers of healthy individuals. Differentially expressed proteins were identified by quantitative proteomic assays. Histopathological findings included neurophagocytosis, microglia nodules, satellite phenomena, extensive edema, focal hemorrhage, and infarction, as well as infiltrating mononuclear cells. Immunostaining of COVID-19 brains revealed extensive activation of both microglia and astrocytes, severe damage of the blood-brain barrier (BBB) and various degrees of perivascular infiltration by predominantly CD14+/CD16+/CD141+/CCR7+/CD11c+ monocytes and occasionally CD4+/CD8+ T lymphocytes. Quantitative proteomic assays combined with bioinformatics analysis identified upregulated proteins predominantly involved in immune responses, autophagy and cellular metabolism in COVID-19 patient brains compared with control brains. Proteins involved in brain development, neuroprotection, and extracellular matrix proteins of the basement membrane were downregulated, potentially caused by the activation of transforming growth factor ß receptor and vascular endothelial growth factor signaling pathways. Thus, our results define histopathological and molecular profiles of COVID-19-associated monocytic encephalitis (CAME) and suggest potential therapeutic targets.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Encephalitis / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Signal Transduct Target Ther Year: 2023 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Encephalitis / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Signal Transduct Target Ther Year: 2023 Document Type: Article