Your browser doesn't support javascript.
Phenotypic changes in low-density lipoprotein particles as markers of adverse clinical outcomes in COVID-19.
Carmo, Helison Rafael P; Yoshinaga, Marcos Y; Castillo, Alejandro Rosell; Britto Chaves-Filho, Adriano; Bonilha, Isabella; Barreto, Joaquim; Muraro, Stéfanie Primon; de Souza, Gabriela Fabiano; Davanzo, Gustavo Gastão; Perroud, Maurício W; Lukhna, Kishal; Ntsekhe, Mpiko; Davidson, Sean; Velloso, Licio A; Nadruz, Wilson; Carvalho, Luiz Sérgio F; Sáinz-Jaspeado, Miguel; Farias, Alessandro S; Proença-Módena, José Luiz; Moraes-Vieira, Pedro M; Karathanasis, Sotirios K; Yellon, Derek; Miyamoto, Sayuri; Remaley, Alan T; Sposito, Andrei C.
  • Carmo HRP; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Yoshinaga MY; Department of Biochemistry, Institute of Chemistry, University of São Paulo, SP, Brazil. Electronic address: marcosyukio@gmail.com.
  • Castillo AR; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Britto Chaves-Filho A; Department of Biochemistry, Institute of Chemistry, University of São Paulo, SP, Brazil.
  • Bonilha I; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Barreto J; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Muraro SP; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
  • de Souza GF; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
  • Davanzo GG; Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
  • Perroud MW; Pneumology Division, Unicamp, SP, Brazil.
  • Lukhna K; Division of Cardiology, University of Cape Town, Cape Town, South Africa.
  • Ntsekhe M; Division of Cardiology, University of Cape Town, Cape Town, South Africa.
  • Davidson S; Hatter Cardiovascular Institute, University College London, London, UK.
  • Velloso LA; Internal Medicine Department, Unicamp Medical School, SP, Brazil.
  • Nadruz W; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Carvalho LSF; Cardiology Division, Unicamp Medical School, SP, Brazil.
  • Sáinz-Jaspeado M; Mercodia AB, Uppsala, Sweden.
  • Farias AS; Laboratory of Neuroimmunomodulation, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil; Hub of Global Health (HGH), University of Campinas, Campinas, SP, Brazil.
  • Proença-Módena JL; Laboratory of Emerging Viruses, Department of Genetics, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil; Hub of Global Health (HGH), University of Campinas, Campinas, SP, Brazil.
  • Moraes-Vieira PM; Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil; Hub of Global Health (HGH), University of Campinas, Campinas, SP, Brazil.
  • Karathanasis SK; Lipoprotein Metabolism Laboratory Translational Vascular Medicine Branch National Heart, Lung and Blood Institute National Institutes of Health, Bethesda, MD, USA.
  • Yellon D; Hatter Cardiovascular Institute, University College London, London, UK.
  • Miyamoto S; Department of Biochemistry, Institute of Chemistry, University of São Paulo, SP, Brazil.
  • Remaley AT; Lipoprotein Metabolism Laboratory Translational Vascular Medicine Branch National Heart, Lung and Blood Institute National Institutes of Health, Bethesda, MD, USA.
  • Sposito AC; Cardiology Division, Unicamp Medical School, SP, Brazil. Electronic address: sposito@unicamp.com.
Mol Genet Metab ; 138(4): 107552, 2023 04.
Article in English | MEDLINE | ID: covidwho-2253818
ABSTRACT
BACKGROUND AND

AIMS:

Low-density lipoprotein (LDL) plasma concentration decline is a biomarker for acute inflammatory diseases, including coronavirus disease-2019 (COVID-19). Phenotypic changes in LDL during COVID-19 may be equally related to adverse clinical outcomes.

METHODS:

Individuals hospitalized due to COVID-19 (n = 40) were enrolled. Blood samples were collected on days 0, 2, 4, 6, and 30 (D0, D2, D4, D6, and D30). Oxidized LDL (ox-LDL), and lipoprotein-associated phospholipase A2 (Lp-PLA2) activity were measured. In a consecutive series of cases (n = 13), LDL was isolated by gradient ultracentrifugation from D0 and D6 and was quantified by lipidomic analysis. Association between clinical outcomes and LDL phenotypic changes was investigated.

RESULTS:

In the first 30 days, 42.5% of participants died due to Covid-19. The serum ox-LDL increased from D0 to D6 (p < 0.005) and decreased at D30. Moreover, individuals who had an ox-LDL increase from D0 to D6 to over the 90th percentile died. The plasma Lp-PLA2 activity also increased progressively from D0 to D30 (p < 0.005), and the change from D0 to D6 in Lp-PLA2 and ox-LDL were positively correlated (r = 0.65, p < 0.0001). An exploratory untargeted lipidomic analysis uncovered 308 individual lipids in isolated LDL particles. Paired-test analysis from D0 and D6 revealed higher concentrations of 32 lipid species during disease progression, mainly represented by lysophosphatidyl choline and phosphatidylinositol. In addition, 69 lipid species were exclusively modulated in the LDL particles from non-survivors as compared to survivors.

CONCLUSIONS:

Phenotypic changes in LDL particles are associated with disease progression and adverse clinical outcomes in COVID-19 patients and could serve as a potential prognostic biomarker.
Subject(s)
Keywords
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: 1-Alkyl-2-acetylglycerophosphocholine Esterase / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Mol Genet Metab Journal subject: Molecular Biology / Biochemistry / Metabolism Year: 2023 Document Type: Article Affiliation country: J.ymgme.2023.107552

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: 1-Alkyl-2-acetylglycerophosphocholine Esterase / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Mol Genet Metab Journal subject: Molecular Biology / Biochemistry / Metabolism Year: 2023 Document Type: Article Affiliation country: J.ymgme.2023.107552