Lipid nanoparticles (LNP) induce activation and maturation of antigen presenting cells in young and aged individuals.
Commun Biol
; 6(1): 188, 2023 02 17.
Article
in English
| MEDLINE | ID: covidwho-2255515
ABSTRACT
Herein, we studied the impact of empty LNP (eLNP), component of mRNA-based vaccine, on anti-viral pathways and immune function of cells from young and aged individuals. eLNP induced maturation of monocyte derived dendritic cells (MDDCs). We further show that eLNP upregulated CD40 and induced cytokine production in multiple DC subsets and monocytes. This coincided with phosphorylation of TANK binding kinase 1 (pTBK1) and interferon response factor 7 (pIRF7). In response to eLNP, healthy older adults (>65 yrs) have decreased CD40 expression, and IFN-γ output compared to young adults (<65 yrs). Additionally, cells from older adults have a dysregulated anti-viral signaling response to eLNP stimulation, measured by the defect in type I IFN production, and phagocytosis. Overall, our data show function of eLNP in eliciting DC maturation and innate immune signaling pathways that is impaired in older adults resulting in lower immune responses to SARS-CoV-2 mRNA-based vaccines.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
COVID-19
Topics:
Vaccines
Limits:
Adult
/
Aged
/
Humans
/
Young adult
Language:
English
Journal:
Commun Biol
Year:
2023
Document Type:
Article
Affiliation country:
S42003-023-04555-1
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