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A simplified viral RNA extraction method based on magnetic nanoparticles for fast and high-throughput detection of SARS-CoV-2.
Cui, Haodong; Song, Wenxing; Ru, Xiaoling; Fu, Wen; Ji, Ling; Zhou, Wenhua; Zhao, Zhen; Qu, Guangbo; Yu, Xue-Feng; Jiang, Guibin.
  • Cui H; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
  • Song W; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China.
  • Ru X; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China; Archean Gene Technology Co., Ltd., Shenzhen, 518107, PR China.
  • Fu W; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
  • Ji L; Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, 518000, PR China.
  • Zhou W; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China. Electronic address: wh.zhou@siat.ac.cn.
  • Zhao Z; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China. Electronic address: zhen.zhao1@siat.ac.cn.
  • Qu G; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China.
  • Yu XF; Materials Interfaces Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, PR China.
  • Jiang G; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China.
Talanta ; 258: 124479, 2023 Jun 01.
Article in English | MEDLINE | ID: covidwho-2255880
ABSTRACT
The ongoing outbreak of the novel coronavirus disease 2019 (COVID-19) draws worldwide concerns due to its long incubation period and strong infectivity. Although RT-PCR-based methods are being widely applied for clinical diagnosis, timely and accurate diagnosis towards COVID-19 causing virus, the SARS-CoV-2, is still limited due to labor-intensive and time-consuming operations. Herein, we report a new viral RNA extraction method based on poly-(amino ester) with carboxyl group (PC)-coated magnetic nanoparticles (pcMNPs) for the sensitive detection of SARS-CoV-2. This method combines the lysis and binding steps into one step, and refines multiple washing steps into one step, giving a turnaround time of less than 9 min. Furthermore, the extracted pcMNP-RNA complexes can be directly introduced into subsequent RT-PCR reactions without elution. This simplified viral RNA method could be well adapted in fast manual and automated high-throughput nucleic acids extraction protocols suitable for different scenarios. A high sensitivity down to 100 copies/mL and a linear correlation between 100 and 106 copies/mL of SARS-CoV-2 pseudovirus particles are achieved in both protocols. Benefitting from the simplicity and excellent performances, this new method can dramatically improve the efficiency and reduce operational requirements for the early clinical diagnosis and large-scale SARS-CoV-2 nucleic acid screening.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetite Nanoparticles / SARS-CoV-2 Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Talanta Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetite Nanoparticles / SARS-CoV-2 Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Talanta Year: 2023 Document Type: Article