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Total and subgenomic RNA viral load in patients infected with SARS-CoV-2 Alpha, Delta, and Omicron variants.
Dimcheff, Derek E; Blair, Christopher N; Zhu, Yuwei; Chappell, James D; Gaglani, Manjusha; McNeal, Tresa; Ghamande, Shekhar; Steingrub, Jay S; Shapiro, Nathan I; Duggal, Abhijit; Busse, Laurence W; Frosch, Anne E P; Peltan, Ithan D; Hager, David N; Gong, Michelle N; Exline, Matthew C; Khan, Akram; Wilson, Jennifer G; Qadir, Nida; Ginde, Adit A; Douin, David J; Mohr, Nicholas M; Mallow, Christopher; Martin, Emily T; Johnson, Nicholas J; Casey, Jonathan D; Stubblefield, William B; Gibbs, Kevin W; Kwon, Jennie H; Talbot, H Keipp; Halasa, Natasha; Grijalva, Carlos G; Baughman, Adrienne; Womack, Kelsey N; Hart, Kimberly W; Swan, Sydney A; Surie, Diya; Thornburg, Natalie J; McMorrow, Meredith L; Self, Wesley H; Lauring, Adam S.
  • Dimcheff DE; Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan.
  • Blair CN; Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan.
  • Zhu Y; Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Chappell JD; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Gaglani M; Baylor Scott and White Health, Texas A&M University College of Medicine, Temple, Texas.
  • McNeal T; Baylor Scott and White Health, Texas A&M University College of Medicine, Temple, Texas.
  • Ghamande S; Baylor Scott and White Health, Texas A&M University College of Medicine, Temple, Texas.
  • Steingrub JS; Department of Medicine, Baystate Medical Center, Springfield, Massachusetts.
  • Shapiro NI; Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts.
  • Duggal A; Department of Medicine, Cleveland Clinic, Cleveland, Ohio.
  • Busse LW; Department of Medicine, Emory University, Atlanta, Georgia.
  • Frosch AEP; Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota.
  • Peltan ID; Department of Medicine, Intermountain Medical Center, Murray, Utah and University of Utah, Salt Lake City, Utah.
  • Hager DN; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Gong MN; Department of Medicine, Montefiore Health System, Albert Einstein College of Medicine, Bronx, New York.
  • Exline MC; Department of Medicine, The Ohio State University, Columbus, Ohio.
  • Khan A; Department of Medicine, Oregon Health and Sciences University, Portland, Oregon.
  • Wilson JG; Department of Emergency Medicine, Stanford University School of Medicine, Stanford, California.
  • Qadir N; Department of Medicine, University of California-Los Angeles, Los Angeles, California.
  • Ginde AA; Department of Emergency Medicine, University of Colorado School of Medicine, Aurora, Colorado.
  • Douin DJ; Department of Anesthesiology, University of Colorado School of Medicine, Aurora, Colorado.
  • Mohr NM; Department of Emergency Medicine, University of Iowa, Iowa City, Iowa.
  • Mallow C; Department of Medicine, University of Miami, Miami, Florida.
  • Martin ET; School of Public Health, University of Michigan, Ann Arbor, Michigan.
  • Johnson NJ; Department of Emergency Medicine and Division of Pulmonary, Critical Care and Sleep Medicine, University of Washington, Seattle, Washington.
  • Casey JD; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Stubblefield WB; Department of Emergency Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Gibbs KW; Department of Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina.
  • Kwon JH; Department of Medicine, Washington University, St. Louis, Missouri.
  • Talbot HK; Departments of Medicine and Health Policy, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Halasa N; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Grijalva CG; Department of Health Policy, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Baughman A; Department of Emergency Medicine, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Womack KN; Vanderbilt Institute for Clinical and Translational Research, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Hart KW; Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Swan SA; Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Surie D; CDC COVID-19 Response Team, Atlanta, Georgia.
  • Thornburg NJ; CDC COVID-19 Response Team, Atlanta, Georgia.
  • McMorrow ML; CDC COVID-19 Response Team, Atlanta, Georgia.
  • Self WH; Vanderbilt Institute for Clinical and Translational Research and Department of Emergency Medicine and, Vanderbilt University Medical Center, Nashville, Tennessee.
  • Lauring AS; Departments of Internal Medicine and Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan.
J Infect Dis ; 2023 Mar 08.
Article in English | MEDLINE | ID: covidwho-2257228
ABSTRACT

BACKGROUND:

SARS-CoV-2 genomic and subgenomic RNA levels are frequently used as a correlate of infectiousness. The impact of host factors and SARS-CoV-2 lineage on RNA viral load is unclear.

METHODS:

Total nucleocapsid (N) and subgenomic N (sgN) RNA levels were measured by RT-qPCR in specimens from 3,204 individuals hospitalized with COVID-19 at 21 hospitals. RT-qPCR cycle threshold (Ct) values were used to estimate RNA viral load. The impact of time of sampling, SARS-CoV-2 variant, age, comorbidities, vaccination, and immune status on N and sgN Ct values were evaluated using multiple linear regression.

RESULTS:

Ct values at presentation for N (mean ±standard deviation) were 24.14±4.53 for non-variants of concern, 25.15±4.33 for Alpha, 25.31±4.50 for Delta, and 26.26±4.42 for Omicron. N and sgN RNA levels varied with time since symptom onset and infecting variant but not with age, comorbidity, immune status, or vaccination. When normalized to total N RNA, sgN levels were similar across all variants.

CONCLUSIONS:

RNA viral loads were similar among hospitalized adults, irrespective of infecting variant and known risk factors for severe COVID-19. Total N and subgenomic RNA N viral loads were highly correlated, suggesting that subgenomic RNA measurements adds little information for the purposes of estimating infectivity.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Language: English Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Language: English Year: 2023 Document Type: Article