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Recent advances in point of care testing for COVID-19 detection.
Fernandes, Renata Salgado; de Oliveira Silva, Juliana; Gomes, Karina Braga; Azevedo, Ricardo Bentes; Townsend, Danyelle M; de Paula Sabino, Adriano; Branco de Barros, Andre Luis.
  • Fernandes RS; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • de Oliveira Silva J; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Gomes KB; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Azevedo RB; Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.
  • Townsend DM; Department of Drug Discovery and Pharmaceutical Sciences, Medical University of South Carolina, USA.
  • de Paula Sabino A; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Branco de Barros AL; Department of Clinical and Toxicological Analyses, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: brancodebarros@yahoo.com.br.
Biomed Pharmacother ; 153: 113538, 2022 Sep.
Article in English | MEDLINE | ID: covidwho-2259011
ABSTRACT
The World Health Organizations declaration of the COVID-19 pandemic was a milestone for the scientific community. The high transmission rate and the huge number of deaths, along with the lack of knowledge about the virus and the evolution of the disease, stimulated a relentless search for diagnostic tests, treatments, and vaccines. The main challenges were the differential diagnosis of COVID-19 and the development of specific, rapid, and sensitive tests that could reach all people. RT-PCR remains the gold standard for diagnosing COVID-19. However, new methods, such as other molecular techniques and immunoassays emerged. Also, the need for accessible tests with quick results boosted the development of point of care tests (POCT) that are fast, and automated, with high precision and accuracy. This assay reduces the dependence on laboratory conditions and mass testing of the population, dispersing the pressure regarding screening and detection. This review summarizes the advances in the diagnostic field since the pandemic started, emphasizing various laboratory techniques for detecting COVID-19. We reviewed the main existing diagnostic methods, as well as POCT under development, starting with RT-PCR detection, but also exploring other nucleic acid techniques, such as digital PCR, loop-mediated isothermal amplification-based assay (RT-LAMP), clustered regularly interspaced short palindromic repeats (CRISPR), and next-generation sequencing (NGS), and immunoassay tests, and nanoparticle-based biosensors, developed as portable instruments for the rapid standard diagnosis of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Biomed Pharmacother Year: 2022 Document Type: Article Affiliation country: J.biopha.2022.113538

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Biomed Pharmacother Year: 2022 Document Type: Article Affiliation country: J.biopha.2022.113538