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Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection.
Gao, Fei; Mallajoysula, Vamsee; Arunachalam, Prabhu S; van der Ploeg, Kattria; Manohar, Monali; Röltgen, Katharina; Yang, Fan; Wirz, Oliver; Hoh, Ramona; Haraguchi, Emily; Lee, Ji-Yeun; Willis, Richard; Ramachandiran, Vasanthi; Li, Jiefu; Kathuria, Karan Raj; Li, Chunfeng; Lee, Alexandra S; Shah, Mihir M; Sindher, Sayantani B; Gonzalez, Joseph; Altman, John D; Wang, Taia T; Boyd, Scott D; Pulendran, Bali; Jagannathan, Prasanna; Nadeau, Kari C; Davis, Mark M.
  • Gao F; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Mallajoysula V; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Arunachalam PS; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • van der Ploeg K; Department of Medicine, Division of Infectious Diseases, Stanford University, Stanford, CA, USA.
  • Manohar M; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Röltgen K; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Yang F; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Wirz O; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Hoh R; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Haraguchi E; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Lee JY; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Willis R; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA.
  • Ramachandiran V; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA.
  • Li J; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Kathuria KR; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Li C; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • Lee AS; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Shah MM; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Sindher SB; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Gonzalez J; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
  • Altman JD; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
  • Wang TT; Department of Medicine, Division of Infectious Diseases, Stanford University, Stanford, CA, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
  • Boyd SD; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
  • Pulendran B; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
  • Jagannathan P; Department of Medicine, Division of Infectious Diseases, Stanford University, Stanford, CA, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.
  • Nadeau KC; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA; Sean N. Parker Center for Allergy and Asthma Research, Stanford University and Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanfo
  • Davis MM; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA. Electronic address: mmdavis@s
Immunity ; 56(4): 864-878.e4, 2023 04 11.
Article in English | MEDLINE | ID: covidwho-2260018
ABSTRACT
T cells are a critical component of the response to SARS-CoV-2, but their kinetics after infection and vaccination are insufficiently understood. Using "spheromer" peptide-MHC multimer reagents, we analyzed healthy subjects receiving two doses of the Pfizer/BioNTech BNT162b2 vaccine. Vaccination resulted in robust spike-specific T cell responses for the dominant CD4+ (HLA-DRB1∗1501/S191) and CD8+ (HLA-A∗02/S691) T cell epitopes. Antigen-specific CD4+ and CD8+ T cell responses were asynchronous, with the peak CD4+ T cell responses occurring 1 week post the second vaccination (boost), whereas CD8+ T cells peaked 2 weeks later. These peripheral T cell responses were elevated compared with COVID-19 patients. We also found that previous SARS-CoV-2 infection resulted in decreased CD8+ T cell activation and expansion, suggesting that previous infection can influence the T cell response to vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Immunity Journal subject: Allergy and Immunology Year: 2023 Document Type: Article Affiliation country: J.immuni.2023.03.005

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Immunity Journal subject: Allergy and Immunology Year: 2023 Document Type: Article Affiliation country: J.immuni.2023.03.005