Chemical Control of CRISPR Gene Editing via Conditional Diacylation Crosslinking of Guide RNAs.
Adv Sci (Weinh)
; 10(10): e2206433, 2023 04.
Article
in English
| MEDLINE | ID: covidwho-2263412
ABSTRACT
Conditional control of RNA structure and function has emerged as an effective toolkit. Here, a strategy based on a one-step introduction of diacylation linkers and azide groups on the 2'-OH of RNA is advance. Selected from eight phosphine reagents, it is found that 2-(diphenylphosphino)ethylamine has excellent performance in reducing azides via a Staudinger reduction to obtain the original RNA. It is demonstrated that the enzymatic activities of Cas13 and Cas9 can be regulated by chemically modified guide RNAs, and further achieved ligand-induced gene editing in living cells by a controllable CRISPR/Cas9 system.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
CRISPR-Cas Systems
/
Gene Editing
Type of study:
Experimental Studies
/
Randomized controlled trials
Language:
English
Journal:
Adv Sci (Weinh)
Year:
2023
Document Type:
Article
Affiliation country:
Advs.202206433
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