Preparation of Zn-Gly and Se-Gly and Their Effects on the Nutritional Quality of Tea (Camellia sinensis).

ABSTRACT

BACKGROUND: Micronutrient malnutrition affects millions of people due to a lack of Zn and Se. METHODS: The process conditions for the manufacture of glycine-chelated sodium selenite (Se-Gly) and zinc sulfate heptahydrate (Zn-Gly) were studied. The effects of ligand concentration, pH, reaction ratio, reaction temperature, and reaction time on fertilizer stability were assessed. The effects of Zn-Gly and Se-Gly on tea plants were determined. RESULTS: Orthogonal experiments showed that the optimal preparation conditions for Zn-Gly (75.80 % Zn chelation rate) were pH 6.0, ligand concentration 4 %, reaction ratio 12, reaction time 120 min, reaction temperature 70 ℃. The optimal preparation conditions for Se-Gly (56.75 % Se chelation rate) were pH 6.0, ligand concentration 10%, reaction ratio 21, reaction time 40 min, temperature 50 ℃. Each chelate was completely soluble in water and verified by infrared spectroscopy and ultraviolet spectroscopy. CONCLUSIONS: Zn-Gly and Se-Gly increased the Zn and Se content in tea plants, and foliar application was more effective than soil application. Combined application of Zn-Gly and Se-Gly was more effective than Zn-Gly or Se-Gly alone. Our findings suggest that Zn-Gly and Se-Gly provide a convenient method of addressing human Zn and Se deficiency.