Targeted capture enrichment and sequencing identifies HLA variants associated with the severity of COVID-19.
Genes Genomics
; 45(4): 451-456, 2023 04.
Article
in English
| MEDLINE | ID: covidwho-2269272
ABSTRACT
BACKGROUND:
Coronavirus disease 2019 (COVID-19) is currently a global pandemic. The pathogenesis of severe COVID-19 has been widely investigated, but it is still unclear. Human leukocyte antigen (HLA) plays a central role in immune response, and its variants might be related to COVID-19 progression and severity.OBJECTIVE:
To investigate the hypothesis that individual HLA variations could alter the course of COVID-19 and might be associated with the severity of COVID-19.METHODS:
In this study, we conducted an HLA targeted capture enrichment and sequencing of severe COVID-19 patients matched to mild cases. A total of 16 COVID-19 patients, confirmed by SARS-CoV-2 viral RNA polymerase-chain-reaction (PCR) test and chest computed tomography (CT) scan, were enrolled in this study. The HLA targeted capture enrichment and sequencing were conducted. HLA typing was performed by comparing contigs with IPD-IMGT/HLA Database.RESULTS:
In this study, 139 four-digit resolution HLA alleles were acquired. The results showed that HLA-DRB3*0101 allele was significantly associated with the severity of COVID-19 (odds ratio [OR] = 27.64, 95% confidence interval [CI] = 1.35-560.50, P = 0.0064). And HLA-K*0101 might be a potential risk factor for COVID-19 severity (OR = 0.11, 95% CI = 0.017-0.66, P = 0.019), but HLA-K*0102 might be a protective factor (OR = 7.50, 95% CI = 1.48-37.92, P = 0.019).CONCLUSION:
Three non-classical HLA alleles, including HLA-DRB3*0101, HLA-K*0101, HLA-K*0102 were identified to be associated with the severity of COVID-19 by comparing mild and severe patients. The current findings would be helpful for exploring the influence of HLA gene polymorphisms on the development and severity of COVID-19.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
COVID-19
Type of study:
Etiology study
/
Prognostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
Genes Genomics
Year:
2023
Document Type:
Article
Affiliation country:
S13258-022-01358-2
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