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Histopathology and SARS-CoV-2 Cellular Localization in Eye Tissues of COVID-19 Autopsies.
Sen, H Nida; Vannella, Kevin M; Wang, Yujuan; Chung, Joon-Yong; Kodati, Shilpa; Ramelli, Sabrina C; Lee, Jung Wha; Perez, Paola; Stein, Sydney R; Grazioli, Alison; Dickey, James M; Ylaya, Kris; Singh, Manmeet; Yinda, Kwe Claude; Platt, Andrew; Ramos-Benitez, Marcos J; Zerbe, Christa; Munster, Vincent J; de Wit, Emmie; Warner, Blake M; Herr, Daniel L; Rabin, Joseph; Saharia, Kapil K; Kleiner, David E; Hewitt, Stephen M; Chan, Chi-Chao; Chertow, Daniel S.
  • Sen HN; National Eye Institute, National Institutes of Health, Bethesda, Maryland.
  • Vannella KM; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
  • Wang Y; National Eye Institute, National Institutes of Health, Bethesda, Maryland.
  • Chung JY; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
  • Kodati S; National Eye Institute, National Institutes of Health, Bethesda, Maryland.
  • Ramelli SC; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland; Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland.
  • Lee JW; National Eye Institute, National Institutes of Health, Bethesda, Maryland.
  • Perez P; Salivary Disorders Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
  • Stein SR; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
  • Grazioli A; Department of Medicine, R Adams Crowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, Maryland.
  • Dickey JM; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
  • Ylaya K; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
  • Singh M; Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
  • Yinda KC; Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
  • Platt A; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
  • Ramos-Benitez MJ; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Postdoctoral Research A
  • Zerbe C; Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
  • Munster VJ; Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
  • de Wit E; Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
  • Warner BM; Salivary Disorders Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
  • Herr DL; Department of Medicine, R Adams Crowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, Maryland.
  • Rabin J; Department of Surgery and Program in Trauma, R Adams Crowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, Maryland.
  • Saharia KK; Department of Medicine, Division of Infectious Disease, University of Maryland School of Medicine, Baltimore, Maryland.
  • Kleiner DE; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
  • Hewitt SM; Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
  • Chan CC; National Eye Institute, National Institutes of Health, Bethesda, Maryland.
  • Chertow DS; the Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland; the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. Electronic address: che
Am J Pathol ; 2023 Mar 22.
Article in English | MEDLINE | ID: covidwho-2274189
ABSTRACT
Ophthalmic manifestations and tissue tropism of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been reported in association with coronavirus disease 2019 (COVID-19), but the pathology and cellular localization of SARS-CoV-2 are not well characterized. The objective of this study was to evaluate macroscopic and microscopic changes and investigate cellular localization of SARS-CoV-2 across ocular tissues at autopsy. Ocular tissues were obtained from 25 patients with COVID-19 at autopsy. SARS-CoV-2 nucleocapsid gene RNA was previously quantified by droplet digital PCR from one eye. Herein, contralateral eyes from 21 patients were fixed in formalin and subject to histopathologic examination. Sections of the droplet digital PCR-positive eyes from four other patients were evaluated by in situ hybridization to determine the cellular localization of SARS-CoV-2 spike gene RNA. Histopathologic abnormalities, including cytoid bodies, vascular changes, and retinal edema, with minimal or no inflammation in ocular tissues were observed in all 21 cases evaluated. In situ hybridization localized SARS-CoV-2 RNA to neuronal cells of the retinal inner and outer layers, ganglion cells, corneal epithelia, scleral fibroblasts, and oligodendrocytes of the optic nerve. In conclusion, a range of common histopathologic alterations were identified within ocular tissue, and SARS-CoV-2 RNA was localized to multiple cell types. Further studies will be required to determine whether the alterations observed were caused by SARS-CoV-2 infection, the host immune response, and/or preexisting comorbidities.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Language: English Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Language: English Year: 2023 Document Type: Article