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Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications.
Picazo-Bueno, José Ángel; Sanz, Martín; Granero, Luis; García, Javier; Micó, Vicente.
  • Picazo-Bueno JÁ; Department of Optics, Optometry and Vision Science, University of Valencia, 46100 Burjassot, Spain.
  • Sanz M; Biomedical Technology Center of the Medical Faculty, University of Muenster, Mendelstr. 17, D-48149 Muenster, Germany.
  • Granero L; Department of Optics, Optometry and Vision Science, University of Valencia, 46100 Burjassot, Spain.
  • García J; Department of Optics, Optometry and Vision Science, University of Valencia, 46100 Burjassot, Spain.
  • Micó V; Department of Optics, Optometry and Vision Science, University of Valencia, 46100 Burjassot, Spain.
Sensors (Basel) ; 23(3)2023 Jan 28.
Article in English | MEDLINE | ID: covidwho-2276447
ABSTRACT
Lensless holographic microscopy (LHM) comes out as a promising label-free technique since it supplies high-quality imaging and adaptive magnification in a lens-free, compact and cost-effective way. Compact sizes and reduced prices of LHMs make them a perfect instrument for point-of-care diagnosis and increase their usability in limited-resource laboratories, remote areas, and poor countries. LHM can provide excellent intensity and phase imaging when the twin image is removed. In that sense, multi-illumination single-holographic-exposure lensless Fresnel (MISHELF) microscopy appears as a single-shot and phase-retrieved imaging technique employing multiple illumination/detection channels and a fast-iterative phase-retrieval algorithm. In this contribution, we review MISHELF microscopy through the description of the principles, the analysis of the performance, the presentation of the microscope prototypes and the inclusion of the main biomedical applications reported so far.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Holography / Lenses Topics: Vaccines Language: English Year: 2023 Document Type: Article Affiliation country: S23031472

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Holography / Lenses Topics: Vaccines Language: English Year: 2023 Document Type: Article Affiliation country: S23031472