Diagnosis of COVID-19 by serology in admitted patients with negative RT-PCR assay
Iranian Journal of Allergy, Asthma and Immunology
; 20(4):394-401, 2021.
Article
in English
| CAB Abstracts | ID: covidwho-2278218
ABSTRACT
Considering the increasing prevalence and burden of coronavirus disease 2019 (COVID-19) disease and false-negative results in routine reverse transcription-polymerase chain reaction (RT-PCR) tests, additional diagnostic methods are needed to diagnose active cases of this disease. This prospective study was conducted on patients, in whom clinical and radiological symptoms/signs were in favor of COVID-19 while their first PCR test was negative. Later on, a second RT-PCR was performed and serological evaluation was carried out and results were compared with each other. Out of 707 patients who had been referred to the hospital and were clinically and radiologically suspicious of disease, 137 patients with negative RT-PCR tests entered the study. RT-PCR assay became positive for the second time in 45 (32.8%). Anti-COVID-19 IgM and IgG antibodies were positive in 83 (60.6%) and 86 (62.8%) patients, respectively. Finally, it was determined that serological test was diagnostic in 73% of patients and the diagnostic yield of serology was significantly higher after the first week of illness (54.8% in the first week and 88% after that). Taking advantage of both serological tests and RT-PCR helps in diagnosing 83.9% of cases. Based on the present study, the serology may be useful as a complementary test and in parallel to RT-PCR assay for diagnosis of COVID-19 among admitted symptomatic cases.
Diagnosis of Human Disease [VV720], Techniques and Methodology [ZZ900], Prion; Viral; Bacterial and Fungal Pathogens of Humans [VV210], reverse transcriptase PCR, diagnostic techniques, diagnosis, human diseases, disease incidence, disease prevalence, epidemiology, Severe acute respiratory syndrome coronavirus 2, man, Iran, Severe acute respiratory syndrome-related coronavirus, Betacoronavirus, Coronavirinae, Coronaviridae, Nidovirales, positive-sense ssRNA Viruses, ssRNA Viruses, RNA Viruses, viruses, Homo, Hominidae, primates, mammals, vertebrates, Chordata, animals, eukaryotes, high Human Development Index countries, lower-middle income countries, Middle East, West Asia, Asia, prospective studies, reverse transcriptase polymerase chain reaction, RT-PCR, SARS-CoV-2
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
English
Journal:
Iranian Journal of Allergy, Asthma and Immunology
Year:
2021
Document Type:
Article
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