Complete genome sequencing and molecular genetic evolution analysis of Guangxi mutant of porcine epidemic diarrhea virus
Genomics and Applied Biology
; 41(8):1692-1702, 2022.
Article
in English, Chinese
| CAB Abstracts | ID: covidwho-2280669
ABSTRACT
In order to understand the genomic characteristics and molecular genetic diversity of porcine epidemic diarrhea virus(PEDV) in Guangxi in recent years, 11 pairs of specific primers were designed to detect the whole genome of PEDV GXNN isolated from porcine diarrhea in Nanning, Guangxi, China, and similarity comparison, genetic evolution, gene variation and S gene recombination were also analyzed. The results showed that full length of the GXNN strain was 28 035 bp, had similar genomic characteristics with other PEDV isolates, about 96.4%-98.7% nucleotide similarity with different reference strains, and the nucleotide similarity of S, ORF3, M and N genes was 93.7%-98.9%, 90.9%-99.4%, 97.4%-99.7% and 95.6%-99.2%;the amino acid similarity of them was 92.9%-99.5%, 91.3%-99.1%, 97.4%-99.1% and 96.4%-99.5%. GXNN is closely related to most domestic isolates in recent years. Phylogenetic tree showed that GXNN closely related to most strains isolated in China recent years, belonged to GII-b subtype. However, it was low relatedness to classic vaccine strains, domestic early epidemic strains, foreign epidemic strains and Guangxi CH-GX-2015-750 A, they belong to different subtypes. Compared with the 5 vaccine strains, the S gene of GXNN stain has a large variation, by inserting amino acid Q at positions 118 844 and 905 sizes, four unique amino acid mutations in the core neutralizing epitope(COE)region and the main epitope region, and 14 mutations in other regions. 126 T/A, 199 A/V and 103 T/A site mutations of ORF3, M and N genes were happened at position 126, 4 D4 region and PN-D4 region, respectively. Recombination analysis revealed that there was a potential recombination region in the hypervariable region of S gene at 826-3 142 nt. This study successfully obtained the complete genome sequence of a PEDV strain, and analyzed its genetic variation and provided a reference for PEDV molecular epidemiology research and new vaccine development.
Genetics and Molecular Biology of Microorganisms [ZZ395], Molecular Biology and Molecular Genetics [ZZ360], Prion; Viral; Bacterial and Fungal Pathogens of Animals [LL821], Taxonomy and Evolution [ZZ380], Animal Immunology [LL650], Host Resistance and Immunity [HH600], genes, genetic diversity, nucleotide sequences, genomes, genome analysis, mutants, amino acid sequences, mutations, open reading frames, molecular epidemiology, vaccine development, Porcine epidemic diarrhea virus, pigs, China, Guangxi, APEC countries, East Asia, Asia, high Human Development Index countries, upper-middle income countries, Central Southern China, Alphacoronavirus, Coronavirinae, Coronaviridae, Nidovirales, positive-sense ssRNA Viruses, ssRNA Viruses, RNA Viruses, viruses, Sus scrofa, Sus, Suidae, Suiformes, Artiodactyla, mammals, vertebrates, Chordata, animals, eukaryotes, isolates, People's Republic of China, DNA sequences, Kwangsi, Porcine epidemic diarrhoea virus, swine, hogs, protein sequences, ORFs
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Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
English
/
Chinese
Journal:
Genomics and Applied Biology
Year:
2022
Document Type:
Article
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