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SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus.
Wang, Xiwen; Li, Song; Qu, Han; Hao, Liangyu; Shao, Ting; Wang, Kai; Xia, Zhiping; Li, Zhiping; Li, Qianxue.
  • Wang X; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
  • Li S; Administration for Drug and Instrument Supervision and Inspection of PLAJLSF Beijing China.
  • Qu H; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
  • Hao L; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
  • Shao T; Jilin Province Animal Husbandry and Veterinary Academy of Sciences Changchun Jilin China.
  • Wang K; The People's Hospital of Changchun Changchun China.
  • Xia Z; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
  • Li Z; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
  • Li Q; Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
Influenza Other Respir Viruses ; 17(3): e13114, 2023 03.
Article in English | MEDLINE | ID: covidwho-2286196
ABSTRACT
The surface-enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10-6 TCID50/ml. The method showed excellent specificity with no cross-reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza, Human / Influenza A Virus, H1N1 Subtype / Influenza A Virus, H5N1 Subtype / Influenza A Virus, H9N2 Subtype / Influenza in Birds Type of study: Diagnostic study / Randomized controlled trials Limits: Animals / Humans Language: English Journal: Influenza Other Respir Viruses Journal subject: Virology Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza, Human / Influenza A Virus, H1N1 Subtype / Influenza A Virus, H5N1 Subtype / Influenza A Virus, H9N2 Subtype / Influenza in Birds Type of study: Diagnostic study / Randomized controlled trials Limits: Animals / Humans Language: English Journal: Influenza Other Respir Viruses Journal subject: Virology Year: 2023 Document Type: Article