Amplification-Free COVID-19 Detection by Digital Droplet REVEALR.
ACS Synth Biol
; 12(4): 1331-1338, 2023 04 21.
Article
in English
| MEDLINE | ID: covidwho-2287537
ABSTRACT
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, exposed a pressing need for new public health tools for pathogen detection, disease diagnosis, and viral genotyping. REVEALR (RNA-encoded viral nucleic acid analyte reporter) is an isothermal DNAzyme-based point-of-care diagnostic that functions with a detection limit of â¼10 copies/µL when coupled with a preamplification step and can be utilized for viral genotyping of SARS-CoV-2 variants of concern through base pair mismatch recognition in a competitive binding format. Here, we describe an advanced REVEALR platform, termed digital droplet REVEALR (ddREVEALR), that can achieve direct viral detection and absolute sample quantitation utilizing a signal amplification strategy that relies on chemical modifications, DNAzyme multiplexing, and volume compression. Using an AI-assisted image-based readout, ddREVEALR was found to achieve 95% positive predictive agreement from a set of 20 nasal pharyngeal swabs collected at UCI Medical Center in Orange, California. We propose that the combination of amplification-free and protein-free analysis makes ddREVEALR a promising application for direct viral RNA detection of clinical samples.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
DNA, Catalytic
/
COVID-19
Type of study:
Diagnostic study
/
Prognostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
ACS Synth Biol
Year:
2023
Document Type:
Article
Affiliation country:
Acssynbio.3c00105
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