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B-Cell Receptor Features and Database Establishment in Recovered COVID-19 Patients by Combining 5'-RACE with PacBio Sequencing.
Zhu, Zhu; Wang, Pingzhang; Jia, Xiaodong; Yu, Meng; Yan, Huige; Liu, Lei; Liu, Wanbing; Zheng, Yaqiong; Kou, Guomei; Wang, Jie; Xu, Weiyan; Huang, Jing; Duan, Fugang; Lu, Fengmin; Fu, Ning; Zhang, Ning; Lu, Yingying; Dai, Hui; Zheng, Shangen; Qiu, Xiaoyan.
  • Zhu Z; NHC Key Laboratory of Medical Immunology, Peking University, 100191 Beijing, China.
  • Wang P; Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, 100191 Beijing, China.
  • Jia X; Department of Immunology, School of Basic Medical Sciences, Peking University, 100191 Beijing, China.
  • Yu M; NHC Key Laboratory of Medical Immunology, Peking University, 100191 Beijing, China.
  • Yan H; Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, 100191 Beijing, China.
  • Liu L; Department of Immunology, School of Basic Medical Sciences, Peking University, 100191 Beijing, China.
  • Liu W; Department of Hepatology, Fifth Medical Center of Chinese PLA General Hospital, 100191 Beijing, China.
  • Zheng Y; NHC Key Laboratory of Medical Immunology, Peking University, 100191 Beijing, China.
  • Kou G; Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, 100191 Beijing, China.
  • Wang J; Department of Immunology, School of Basic Medical Sciences, Peking University, 100191 Beijing, China.
  • Xu W; NHC Key Laboratory of Medical Immunology, Peking University, 100191 Beijing, China.
  • Huang J; Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, 100191 Beijing, China.
  • Duan F; Department of Immunology, School of Basic Medical Sciences, Peking University, 100191 Beijing, China.
  • Lu F; Department of Transfusion Medicine, General Hospital of Central Theater Command of PLA, 430000 Wuhan, Hubei, China.
  • Fu N; Department of Transfusion Medicine, General Hospital of Central Theater Command of PLA, 430000 Wuhan, Hubei, China.
  • Zhang N; Department of Transfusion Medicine, General Hospital of Central Theater Command of PLA, 430000 Wuhan, Hubei, China.
  • Lu Y; Department of Transfusion Medicine, General Hospital of Central Theater Command of PLA, 430000 Wuhan, Hubei, China.
  • Dai H; Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, 100191 Beijing, China.
  • Zheng S; NHC Key Laboratory of Medical Immunology, Peking University, 100191 Beijing, China.
  • Qiu X; Key Laboratory of Molecular Immunology, Chinese Academy of Medical Sciences, 100191 Beijing, China.
Front Biosci (Landmark Ed) ; 28(2): 40, 2023 02 28.
Article in English | MEDLINE | ID: covidwho-2289073
ABSTRACT

BACKGROUND:

Antibodies induced by viral infection can not only prevent subsequent virus infection, but can also mediate pathological injury following infection. Therefore, understanding the B-cell receptor (BCR) repertoire of either specific neutralizing or pathological antibodies from patients convalescing from Coronavirus disease 2019 (COVID-19) infection is of benefit for the preparation of therapeutic or preventive antibodies, and may provide insight into the mechanisms of COVID-19 pathological injury.

METHODS:

In this study, we used a molecular approach of combining 5' Rapid Amplification of cDNA Ends (5'-RACE) with PacBio sequencing to analyze the BCR repertoire of all 5 IgH and 2 IgL genes in B-cells harvested from 35 convalescent patients after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

RESULTS:

We observed numerous BCR clonotypes within most COVID-19 patients, but not in healthy controls, which validates the association of the disease with a prototypical immune response. In addition, many clonotypes were found to be frequently shared between different patients or different classes of antibodies.

CONCLUSIONS:

These convergent clonotypes provide a resource to identify potential therapeutic/prophylactic antibodies, or identify antibodies associated with pathological effects following infection with SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Front Biosci (Landmark Ed) Year: 2023 Document Type: Article Affiliation country: J.fbl2802040

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Front Biosci (Landmark Ed) Year: 2023 Document Type: Article Affiliation country: J.fbl2802040