Cholesterol and Melatonin Exert Opposite Effects on the Interaction of Model DMPC Membranes with the SARS-CoV-2 S-Protein: A SANS Study
Macromolecular Symposia
; 408(1), 2023.
Article
in English
| Scopus | ID: covidwho-2292705
ABSTRACT
An effect of receptor-binding domain (RBD) of SARS-CoV-2 S-protein on structural parameters of model lipid membranes presented by dimyristoylphosphatidylcholine (DMPC) systems with cholesterol and melatonin impurities is studied by small angle neutron scattering (SANS). It is shown that an increase in melatonin concentration in the lipid membrane leads to a decrease in the thickness of the lipid bilayer, while an increase in the concentration of cholesterol leads to an increase in its thickness. It is suggested that increasing the concentration of melatonin in a membrane prevents the interaction of coronaviral S-protein with a lipid membrane of a cell. In the presence of cholesterol in the system, the interaction of a lipid membrane with an active part of S-protein occurs depending on a phase state of the lipid in the case of a gel phase, there is no changes in structural parameters, but at higher temperatures in the case of a liquid crystal phase, an addition of RBD SARS-CoV-2 to the system causes a reduce in the membrane thickness. © 2023 Wiley-VCH GmbH.
cholesterol; lipid membrane; melatonin; S-protein; SARS-CoV-2; small-angle neutron scattering; Crystal impurities; Lipid bilayers; Lipids; Liquid crystals; Neutron scattering; Proteins; Active parts; Dimyristoylphosphatidylcholine; Lipid membranes; Phase state; Protein A; Receptor-binding domains; Structural parameter; Coronavirus
Full text:
Available
Collection:
Databases of international organizations
Database:
Scopus
Type of study:
Experimental Studies
Language:
English
Journal:
Macromolecular Symposia
Year:
2023
Document Type:
Article
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