Your browser doesn't support javascript.
Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus.
Chen, Da-Yuan; Turcinovic, Jacquelyn; Feng, Shuchen; Kenney, Devin J; Chin, Chue Vin; Choudhary, Manish C; Conway, Hasahn L; Semaan, Marc; Close, Brianna J; Tavares, Alexander H; Seitz, Scott; Khan, Nazimuddin; Kapell, Sebastian; Crossland, Nicholas A; Li, Jonathan Z; Douam, Florian; Baker, Susan C; Connor, John H; Saeed, Mohsan.
  • Chen DY; Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Turcinovic J; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Feng S; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Kenney DJ; Department of Microbiology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Chin CV; Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA.
  • Choudhary MC; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Conway HL; Department of Microbiology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Semaan M; Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Close BJ; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Tavares AH; Brigham and Women's Hospital, Boston, MA, USA.
  • Seitz S; Harvard Medical School, Cambridge, MA, USA.
  • Khan N; Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Kapell S; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Crossland NA; Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Li JZ; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Douam F; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
  • Baker SC; Department of Microbiology, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Connor JH; Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA.
  • Saeed M; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
iScience ; 26(5): 106634, 2023 May 19.
Article in English | MEDLINE | ID: covidwho-2293245
ABSTRACT
A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Language: English Journal: IScience Year: 2023 Document Type: Article Affiliation country: J.isci.2023.106634

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Topics: Vaccines / Variants Language: English Journal: IScience Year: 2023 Document Type: Article Affiliation country: J.isci.2023.106634