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Development of neutralizing antibodies against SARS-CoV-2, using a high-throughput single-B-cell cloning method.
Dou, Yang; Xu, Ke; Deng, Yong-Qiang; Jia, Zijing; Lan, Jun; Xu, Xiaoyu; Zhang, Guorui; Cao, Tianshu; Liu, Pan; Wang, Xiangxi; Wang, Xinquan; Xu, Lingjie; Du, Pan; Qin, Cheng-Feng; Liu, Hong; Li, Yafeng; Wu, Guizhen; Wang, Kang; Lu, Bai.
  • Dou Y; School of Pharmaceutical Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China.
  • Xu K; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Deng YQ; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China.
  • Jia Z; National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Lan J; Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Xu X; Vazyme Biotech Co., Ltd., Nanjing, China.
  • Zhang G; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
  • Cao T; National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Liu P; National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Wang X; National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Wang X; Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Xu L; Vazyme Biotech Co., Ltd., Nanjing, China.
  • Du P; Vazyme Biotech Co., Ltd., Nanjing, China.
  • Qin CF; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China.
  • Liu H; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
  • Li Y; Shanxi Provincial Key Laboratory of Kidney Disease, The Fifth Hospital of Shanxi Medical University, Taiyuan 030012, China.
  • Wu G; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Wang K; National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Lu B; School of Pharmaceutical Sciences, IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China.
Antib Ther ; 6(2): 76-86, 2023 Apr.
Article in English | MEDLINE | ID: covidwho-2294418
ABSTRACT

Background:

Rapid and efficient strategies are needed to discover neutralizing antibodies (nAbs) from B cells derived from virus-infected patients.

Methods:

Here, we report a high-throughput single-B-cell cloning method for high-throughput isolation of nAbs targeting diverse epitopes on the SARS-CoV-2-RBD (receptor binding domain) from convalescent COVID-19 patients. This method is simple, fast and highly efficient in generating SARS-CoV-2-neutralizing antibodies from COVID-19 patients' B cells.

Results:

Using this method, we have developed multiple nAbs against distinct SARS-CoV-2-RBD epitopes. CryoEM and crystallography revealed precisely how they bind RBD. In live virus assay, these nAbs are effective in blocking viral entry to the host cells.

Conclusion:

This simple and efficient method may be useful in developing human therapeutic antibodies for other diseases and next pandemic.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Antib Ther Year: 2023 Document Type: Article Affiliation country: Abt

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Antib Ther Year: 2023 Document Type: Article Affiliation country: Abt