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A Preliminary Study of Mild Heat Stress on Inflammasome Activation in Murine Macrophages.
Foster, Simmie L; Dutton, Abigail J; Yerzhan, Adina; March, Lindsay B; Barry, Katherine; Seehus, Corey R; Huang, Xudong; Talbot, Sebastien; Woolf, Clifford J.
  • Foster SL; Department of Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
  • Dutton AJ; FM Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Yerzhan A; FM Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • March LB; FM Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Barry K; Department of Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
  • Seehus CR; FM Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Huang X; Department of Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
  • Talbot S; Department of Pharmacology and Physiology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
  • Woolf CJ; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada.
Cells ; 12(8)2023 04 19.
Article in English | MEDLINE | ID: covidwho-2295139
ABSTRACT
Inflammation and mitochondrial-dependent oxidative stress are interrelated processes implicated in multiple neuroinflammatory disorders, including Alzheimer's disease (AD) and depression. Exposure to elevated temperature (hyperthermia) is proposed as a non-pharmacological, anti-inflammatory treatment for these disorders; however, the underlying mechanisms are not fully understood. Here we asked if the inflammasome, a protein complex essential for orchestrating the inflammatory response and linked to mitochondrial stress, might be modulated by elevated temperatures. To test this, in preliminary studies, immortalized bone-marrow-derived murine macrophages (iBMM) were primed with inflammatory stimuli, exposed to a range of temperatures (37-41.5 °C), and examined for markers of inflammasome and mitochondrial activity. We found that exposure to mild heat stress (39 °C for 15 min) rapidly inhibited iBMM inflammasome activity. Furthermore, heat exposure led to decreased ASC speck formation and increased numbers of polarized mitochondria. These results suggest that mild hyperthermia inhibits inflammasome activity in the iBMM, limiting potentially harmful inflammation and mitigating mitochondrial stress. Our findings suggest an additional potential mechanism by which hyperthermia may exert its beneficial effects on inflammatory diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Inflammasomes / NLR Family, Pyrin Domain-Containing 3 Protein Limits: Animals Language: English Year: 2023 Document Type: Article Affiliation country: Cells12081189

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Inflammasomes / NLR Family, Pyrin Domain-Containing 3 Protein Limits: Animals Language: English Year: 2023 Document Type: Article Affiliation country: Cells12081189