Detection of viral antibodies in camel sera using magnetic particle spectroscopy.
Appl Microbiol Biotechnol
; 107(10): 3329-3339, 2023 May.
Article
in English
| MEDLINE | ID: covidwho-2295184
ABSTRACT
Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections. KEY POINTS ⢠COMPASS (critical offset magnetic particle spectroscopy) allows the fast detection of antibodies. ⢠Magnetic nanoparticles can be adapted by exchange of the linked bait molecule. ⢠Antibodies could be detected in camel sera without washing steps within seconds.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Middle East Respiratory Syndrome Coronavirus
/
COVID-19
Type of study:
Diagnostic study
/
Observational study
Limits:
Animals
Language:
English
Journal:
Appl Microbiol Biotechnol
Year:
2023
Document Type:
Article
Affiliation country:
S00253-023-12513-4
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