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Plasma cell-free DNA promise monitoring and tissue injury assessment of COVID-19.
Jin, Xin; Wang, Yanqun; Xu, Jinjin; Li, Yimin; Cheng, Fanjun; Luo, Yuxue; Zhou, Haibo; Lin, Shanwen; Xiao, Fei; Zhang, Lu; Lin, Yu; Zhang, Zhaoyong; Jin, Yan; Zheng, Fang; Chen, Wei; Zhu, Airu; Tao, Ye; Zhao, Jingxian; Kuo, Tingyou; Li, Yuming; Li, Lingguo; Wen, Liyan; Ou, Rijing; Li, Fang; Lin, Long; Zhang, Yanjun; Sun, Jing; Yuan, Hao; Zhuang, Zhen; Sun, Haixi; Chen, Zhao; Li, Jie; Zhuo, Jianfen; Chen, Dongsheng; Zhang, Shengnan; Sun, Yuzhe; Wei, Peilan; Yuan, Jinwei; Xu, Tian; Yang, Huanming; Wang, Jian; Xu, Xun; Zhong, Nanshan; Xu, Yonghao; Sun, Kun; Zhao, Jincun.
  • Jin X; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China. jinxin@genomics.cn.
  • Wang Y; School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China. jinxin@genomics.cn.
  • Xu J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Li Y; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Cheng F; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Luo Y; Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, China.
  • Zhou H; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Lin S; School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China.
  • Xiao F; The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, 511500, Guangdong, China.
  • Zhang L; Yangjiang People's Hospital, Yangjiang, 529500, Guangdong, China.
  • Lin Y; Department of Infectious Diseases, Guangdong Provincial Key Laboratory of Biomedical Imaging, Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 519000, Guangdong Province, China.
  • Zhang Z; Institute of Infectious Disease, Guangzhou Eighth People's Hospital of Guangzhou Medical University, Guangzhou, 510060, Guangdong, China.
  • Jin Y; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Zheng F; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Chen W; Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, China.
  • Zhu A; Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, China.
  • Tao Y; School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China.
  • Zhao J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Kuo T; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Li Y; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Li L; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Wen L; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, Guangdong, China.
  • Ou R; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Li F; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Lin L; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, Guangdong, China.
  • Zhang Y; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Sun J; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Yuan H; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Zhuang Z; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Sun H; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, Guangdong, China.
  • Chen Z; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Li J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Zhuo J; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Chen D; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, Guangdong, China.
  • Zhang S; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Sun Y; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Wei P; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Yuan J; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Xu T; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, Guangdong, China.
  • Yang H; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Wang J; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Xu X; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Zhong N; BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
  • Xu Y; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Sun K; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
  • Zhao J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, Guangdong, China.
Mol Genet Genomics ; 298(4): 823-836, 2023 Jul.
Article in English | MEDLINE | ID: covidwho-2297231
ABSTRACT
Coronavirus 2019 (COVID-19) is a complex disease that affects billions of people worldwide. Currently, effective etiological treatment of COVID-19 is still lacking; COVID-19 also causes damages to various organs that affects therapeutics and mortality of the patients. Surveillance of the treatment responses and organ injury assessment of COVID-19 patients are of high clinical value. In this study, we investigated the characteristic fragmentation patterns and explored the potential in tissue injury assessment of plasma cell-free DNA in COVID-19 patients. Through recruitment of 37 COVID-19 patients, 32 controls and analysis of 208 blood samples upon diagnosis and during treatment, we report gross abnormalities in cfDNA of COVID-19 patients, including elevated GC content, altered molecule size and end motif patterns. More importantly, such cfDNA fragmentation characteristics reflect patient-specific physiological changes during treatment. Further analysis on cfDNA tissue-of-origin tracing reveals frequent tissue injuries in COVID-19 patients, which is supported by clinical diagnoses. Hence, our work demonstrates and extends the translational merit of cfDNA fragmentation pattern as valuable analyte for effective treatment monitoring, as well as tissue injury assessment in COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cell-Free Nucleic Acids / COVID-19 Type of study: Diagnostic study / Etiology study / Prognostic study Limits: Humans Language: English Journal: Mol Genet Genomics Journal subject: Molecular Biology / Genetics Year: 2023 Document Type: Article Affiliation country: S00438-023-02014-4

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cell-Free Nucleic Acids / COVID-19 Type of study: Diagnostic study / Etiology study / Prognostic study Limits: Humans Language: English Journal: Mol Genet Genomics Journal subject: Molecular Biology / Genetics Year: 2023 Document Type: Article Affiliation country: S00438-023-02014-4