Comparative analysis of SARS-CoV-2 variants with two high-throughput sequencing platforms
Chinese Journal of Zoonoses
; 38(9):771-777, 2022.
Article
in Chinese
| GIM | ID: covidwho-2298711
ABSTRACT
Whole-genome sequencing of upper respiratory tract specimens from patients with confirmed COVID-19 in Henan Province was performed to compare the performance of the Illumina and Oxford Nanopore sequencing platforms, thus providing a reference for whole-genome monitoring of the novel coronavirus (SARS-CoV-2). Ten samples from COVID-19 cases in Henan Province from June 2021 to January 2022 were collected and sequenced with Illumina and Nanopore high-through-put sequencing technology to obtain full genome sequences of the novel coronavirus, which were compared with the Wuhan reference sequence (Wuhan-Hu-1). Bioinformatics software (CLC) was used for sequence alignment analysis. Three of the ten samples were Omicron (BA.1) variants with 55,61 nucleotide variation sites. One sample was an Alpha (B.1.1.7) variant with 41 nucleotide variation sites. Six samples were Delta (8.1.617.2) variants with 35,42,47 nucleotide variation sites. The sequence identity of mutation sites in six samples was 100%, and the mutation sites in the S genome segment of seven samples were consistent. For samples with a Ct value < 33, both next-generation and third-generation sequencing achieved high genome coverage and sequencing depth. A significant difference in coverage was observed between second-generation sequencing and third-generation sequencing (t=-2.037, P < 0.06). However, the coverage at different time points of the third-generation sequencing did not significantly differ (F=2.498, P > 0.05). The needs for SARS-CoV-2 mutant detection could be met through use of either high-throughput sequencing platform. The identification of mutations in the novel coronavirus through Illumina high-throughput sequencing was more accurate, whereas Nanopore high-throughput sequencing technology could be used for rapid detection and typing of different novel coronaviruses.
viral diseases; upper respiratory tract infections; typing methods; nucleotide sequences; next generation sequencing; mutations; mutational analysis; mutants; molecular genetics techniques; molecular genetics; human diseases; genomes; genome analysis; DNA sequencing; coronavirus disease 2019; bioinformatics; Severe acute respiratory syndrome coronavirus 2; man; Henan; China; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Central Southern China; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; SARS-CoV-2 variants; msn; viral infections; SARS-CoV-2; DNA sequences; biochemical genetics; Honan; nucleotide sequence analysis; nucleotide sequencing; People's Republic of China
Full text:
Available
Collection:
Databases of international organizations
Database:
GIM
Topics:
Variants
Language:
Chinese
Journal:
Chinese Journal of Zoonoses
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS