CRISPR-Cas assisted diagnostics: A broad application biosensing approach
TrAC - Trends in Analytical Chemistry
; 162 (no pagination), 2023.
Article
in English
| EMBASE | ID: covidwho-2299695
ABSTRACT
In addition to its remarkable genome editing capability, the CRISPR-Cas system has proven to be very effective in many fields of application, including the biosensing of pathogenic infections, mutagenic defects, or early cancer diagnosis. Thanks to their many advantages in terms of simplicity, efficiency, and reduced time, several CRISPR-Cas systems have been described for the design of sensitive and selective analytical tools, paving the way for the development and further commercialization of next-generation diagnostics. However, CRISPR-Cas-based biosensors still need further research efforts to improve some drawbacks, such as the need for target amplification, low reproducibility, and lack of knowledge of exploited element robustness. This review aims to describe the latest trends in the design of CRISPR-Cas biosensing technologies to better highlight the insights of their advantages and to point out the limitations that still need to be overcome for their future market entry as medical diagnostics.Copyright © 2023 Elsevier B.V.
Cancer biomarkers; CRISPR-Cas biosensing; Infectious diseases; Nucleic acid amplification; Point of care; adaptive immunity; branched DNA signal amplification assay; cancer diagnosis; coronavirus disease 2019/di [Diagnosis]; CRISPR Cas system; diagnostic procedure; DNA sequence; gene editing; genomics; human; infection/di [Diagnosis]; nanotechnology; point of care testing; real time polymerase chain reaction; recombinase polymerase amplification; review; sensitivity and specificity; severe acute respiratory syndrome/di [Diagnosis]; trend study; virus infection/di [Diagnosis]; cellular apoptosis susceptibility protein/ec [Endogenous Compound]; circulating tumor DNA/ec [Endogenous Compound]; CRISPR associated endonuclease Cas9/ec [Endogenous Compound]; RNA/ec [Endogenous Compound]; tumor marker/ec [Endogenous Compound]; unclassified drug; biosensor; electrochemical biosensor; agro environmental application; hybridization chain reaction; primer exchange reaction; regenerative biosensor; C2c1 protein/ec [Endogenous Compound]; C2c2 protein/ec [Endogenous Compound]; C2c3 protein/ec [Endogenous Compound]; Cas12 protein/ec [Endogenous Compound]; cas13 protein/ec [Endogenous Compound]; Cas3 protein/ec [Endogenous Compound]; Cpf1 protein/ec [Endogenous Compound]; crispr RNA/ec [Endogenous Compound]; csf1 protein/ec [Endogenous Compound]; PIK3CA gene
Full text:
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Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
TrAC - Trends in Analytical Chemistry
Year:
2023
Document Type:
Article
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