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SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests.
Nguyen, Phuong-Vi; Carmola, Ludy Registre; Wang, Ethan; Bassit, Leda; Rao, Anuradha; Greenleaf, Morgan; Sullivan, Julie A; Martin, Greg S; Lam, Wilbur A; Waggoner, Jesse J; Piantadosi, Anne.
  • Nguyen PV; Emory University Department of Medicine, Atlanta, GA, USA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA.
  • Carmola LR; Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA.
  • Wang E; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA; Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA.
  • Bassit L; Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA; Laboratory of Biochemical Pharmacology, Emory University, Atlanta, GA, USA.
  • Rao A; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA; Emory University Department of Pediatrics, Atlanta, GA, USA.
  • Greenleaf M; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA.
  • Sullivan JA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA.
  • Martin GS; Emory University Department of Medicine, Atlanta, GA, USA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA.
  • Lam WA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA; Emory University Department of Pediatrics, Atlanta, GA, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
  • Waggoner JJ; Emory University Department of Medicine, Atlanta, GA, USA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA. Electronic address: jjwaggo@emory.edu.
  • Piantadosi A; Emory University Department of Medicine, Atlanta, GA, USA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA; Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA.
J Clin Virol ; 162: 105426, 2023 05.
Article in English | MEDLINE | ID: covidwho-2300902
ABSTRACT
Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Clin Virol Journal subject: Virology Year: 2023 Document Type: Article Affiliation country: J.jcv.2023.105426

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: J Clin Virol Journal subject: Virology Year: 2023 Document Type: Article Affiliation country: J.jcv.2023.105426