Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease.
Protein Expr Purif
; 207: 106267, 2023 07.
Article
in English
| MEDLINE | ID: covidwho-2302124
ABSTRACT
Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Single-Domain Antibodies
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Protein Expr Purif
Journal subject:
Molecular Biology
Year:
2023
Document Type:
Article
Affiliation country:
J.pep.2023.106267
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