Automatic Analysis of Isothermal Amplification via Impedance Time-Constant-Domain Spectroscopy: A SARS-CoV-2 Case Study
Chemosensors
; 11(4):230, 2023.
Article
in English
| ProQuest Central | ID: covidwho-2302293
ABSTRACT
The development of sensitive and affordable testing devices for infectious diseases is essential to preserve public health, especially in pandemic scenarios. In this work, we have developed an attractive analytical method to monitor products of genetic amplification, particularly the loop-mediated isothermal amplification reaction (RT-LAMP). The method is based on electrochemical impedance measurements and the distribution of relaxation times model, to provide the so-called time-constant-domain spectroscopy (TCDS). The proposed method is tested for the SARS-CoV-2 genome, since it has been of worldwide interest due to the COVID-19 pandemic. Particularly, once the method is calibrated, its performance is demonstrated using real wastewater samples. Moreover, we propose a simple classification algorithm based on TCDS data to discriminate among positive and negative samples. Results show how a TCDS-based method provides an alternative mechanism for label-free and automated assays, exhibiting robustness and specificity for genetic detection.
Chemistry--Physical Chemistry; impedance spectroscopy; electrochemical sensor; distribution of relaxation times; data analytics; isothermal amplification; SARS-CoV-2; Pathogens; Public health; Gene amplification; Severe acute respiratory syndrome coronavirus 2; Epidemiology; Genomes; Infectious diseases; Diagnostic tests; Water sampling; Nanomaterials; COVID-19; Automation; Wastewater; Domains; Pandemics; Spectrum analysis; Sensors; Sensitivity analysis; Spectroscopy; Algorithms; Methods; Biosensors; Screen printing; Coronaviruses; United States--US
Full text:
Available
Collection:
Databases of international organizations
Database:
ProQuest Central
Type of study:
Case report
Language:
English
Journal:
Chemosensors
Year:
2023
Document Type:
Article
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