Optimized rapid production of recombinant secreted proteins in CHO cells grown in suspension: The case of RBD.
Biotechnol Appl Biochem
; 2022 Oct 03.
Article
in English
| MEDLINE | ID: covidwho-2302746
ABSTRACT
Chinese Hamster Ovary cells (CHO) have become the most common workhorse for the commercial production of therapeutic proteins, as well as for the production of recombinant proteins for biomedical research. The ability to grow at high density in suspension, the adaptability to serum free media, and the ease transfection and scale up, made CHO cell line highly productive and robust for large-scale production. Here, we present an optimized workflow used to successfully express and purify a number of human proteins with a yield up to 5 mg/L of culture. The entire protocol, from the synthetic gene design to the assessment of purified protein quality, can be completed in 2 weeks. The established cell culture platform has been efficiently adapted to rapidly produce the receptor-binding domain (RBD) in SARS-CoV-2 S protein, a protein required by many laboratories in 2020 to better understand the initial step of infection related to COVID-19 pandemic. An overall yield of 2 mg of high quality soluble RBD per liter of culture was obtained, a production 10-times cheaper than commercial preparations, this representing an intriguing strategy for future challenges.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal subject:
Biochemistry
/
Biotechnology
Year:
2022
Document Type:
Article
Affiliation country:
Bab.2409
Similar
MEDLINE
...
LILACS
LIS