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Calcitriol modifies tight junctions, improves barrier function, and reduces TNF-α-induced barrier leak in the human lung-derived epithelial cell culture model, 16HBE 14o.
Rybakovsky, Elizabeth; DiGuilio, Katherine M; Valenzano, Mary Carmen; Geagan, Sophie; Pham, Kaithlyn; Harty, Ronald N; Mullin, James M.
  • Rybakovsky E; The Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA.
  • DiGuilio KM; The Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA.
  • Valenzano MC; The Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA.
  • Geagan S; Department of Biology, Drexel University, Philadelphia, Pennsylvania, USA.
  • Pham K; Department of Biology, Drexel University, Philadelphia, Pennsylvania, USA.
  • Harty RN; Department of Pathobiology and Microbiology, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, Pennsylvania, USA.
  • Mullin JM; The Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA.
Physiol Rep ; 11(7): e15592, 2023 04.
Article in English | MEDLINE | ID: covidwho-2302852
ABSTRACT
Using the 16HBE 14o- human airway epithelial cell culture model, calcitriol (Vitamin D) was shown to improve barrier function by two independent metrics - increased transepithelial electrical resistance (TER) and reduced transepithelial diffusion of 14 C-D-mannitol (Jm ). Both effects were concentration dependent and active out to 168 h post-treatment. Barrier improvement associated with changes in the abundance of specific tight junctional (TJ) proteins in detergent-soluble fractions, most notably decreased claudin-2. TNF-α-induced compromise of barrier function could be attenuated by calcitriol with a concentration dependence similar to that observed for improvement of control barrier function. TNF-α-induced increases in claudin-2 were partially reversed by calcitriol. The ERK 1,2 inhibitor, U0126, itself improved 16HBE barrier function indicating MAPK pathway regulation of 16HBE barrier function. Calcitriol's action was additive to the effect of U0126 in reducing TNF- α -induced barrier compromise, suggesting that calcitriol may be acting through a non-ERK pathway in its blunting of TNF- α - induced barrier compromise. This was supported by calcitriol being without effect on pERK levels elevated by the action of TNF-α. Lack of effect of TNF- α on the death marker, caspase-3, and the inability of calcitriol to decrease the elevated LC3B II level caused by TNF-α, suggest that calcitriol's barrier improvement does not involve a cell death pathway. Calcitriol's improvement of control barrier function was not additive to barrier improvement induced by retinoic acid (Vitamin A). Calcitriol improvement and protection of airway barrier function could in part explain Vitamin D's reported clinical efficacy in COVID-19 and other airway diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tumor Necrosis Factor-alpha / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Physiol Rep Year: 2023 Document Type: Article Affiliation country: Phy2.15592

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tumor Necrosis Factor-alpha / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Physiol Rep Year: 2023 Document Type: Article Affiliation country: Phy2.15592