Your browser doesn't support javascript.
New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging.
Sankiewicz, Anna; Zelazowska-Rutkowska, Beata; Gorska, Ewelina; Hermanowicz, Adam; Gorodkiewicz, Ewa.
  • Sankiewicz A; Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, Poland.
  • Zelazowska-Rutkowska B; Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, Poland.
  • Gorska E; Independent Researcher, Stoleczna 7, 15-879 Bialystok, Poland.
  • Hermanowicz A; Department of Pediatric Surgery and Urology, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, Poland.
  • Gorodkiewicz E; Department of Pediatric Surgery and Urology, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, Poland.
Sensors (Basel) ; 23(8)2023 Apr 19.
Article in English | MEDLINE | ID: covidwho-2303077
ABSTRACT
Neuropilin-1 is transmembrane protein with soluble isoforms. It plays a pivotal role in both physiological and pathological processes. NRP-1 is involved in the immune response, formation of neuronal circuits, angiogenesis, survival and migration of cells. The specific SPRI biosensor for the determination of neuropilin-1 was constructed using mouse monoclonal antibody that captures unbound NRP-1 form body fluids. The biosensor exhibits linearity of the analytical signal between 0.01 and 2.5 ng/mL, average precision value 4.7% and recovery between 97% and 104%. The detection limit is 0.011 ng/mL, and the limit of quantification is 0.038 ng/mL. The biosensor was validated by parallel determination of NRP-1 in serum and saliva samples using the ELISA test, with good agreement of the results.
Subject(s)
Keywords
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Surface Plasmon Resonance Type of study: Prognostic study Limits: Animals Language: English Year: 2023 Document Type: Article Affiliation country: S23084118

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Surface Plasmon Resonance Type of study: Prognostic study Limits: Animals Language: English Year: 2023 Document Type: Article Affiliation country: S23084118